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Trivedi, M. K., Branton, A., Trivedi, D., Nayak, G., Afaganis, A. E., Bader, B. M., et al. (2017). Differential Regulation of Immune Biomarkers by the Trivedi Effect®- Energy of Consciousness Healing Treatment Based Herbomineral Formulation in Male Sprague Dawley Rats. Biochemistry and Molecular Biology, 2(6). Retrieved December 16, 2019, from http://dx.doi.org/10.11648/j.bmb.20170206.18
Abstract: A new proprietary herbomineral formulation was formulated, consisting of essential ingredients viz. herbal root extract of ashwagandha and minerals (zinc, magnesium, and selenium). The present study aimed to evaluate the impact of The Trivedi Effect®- Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) based herbomineral formulation in male Sprague Dawley (SD) rats for immune biomarkers modulation. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Treated sample, which received the Biofield Energy Healing Treatment remotely from twenty renowned Biofield Energy Healers. The immunomodulatory potential was studied in male SD rats using various immune biomarkers such as IgG and IgM, CD4+ and CD8+, hematology, lipid profile, hepatic enzymes, sex hormone, and antioxidant profile. The humoral immune response showed an increase IgG level by 6.10% in the Biofield Energy Treated test formulation (G4) group compared with the disease control (G2) group. Cellular immune response i.e. CD4+ and CD8+ counts were significantly improved by 51.64% and 52.09%, respectively in G4 compared with the G2 group. The lymphocytes level were increased by 38.87%, while TLC, neutrophils, eosinophils, and monocytes count were decreased by 7.38%, 19.35%, 21.14%, and 3.43%, respectively in G4 compared with the G2 group. Lipid analysis showed that triglycerides and VLDL levels were decreased by 6.02% and 5.97%, respectively while HDL level was significantly increased by 28.44% in G4 compared with the G2 group. Hepatic biomarkers viz. SGOT, CK-MB, total protein, total bilirubin, and globulin by 6.27%, 14.97%, 2.85%, 16.66%, and 5.90%, respectively in G4 compared with the G2 group. However, the testosterone level was significantly increased in G4 and untreated test formulation (G5) groups by 135.74% and 28.09%, respectively compared with the G2 group. Additionally, the antioxidant assay showed that the lipid peroxidation (LPO) level was decreased, while superoxide dismutase (SOD) and catalase levels were increased by 59.72% and 20.07%, respectively in the G4 compared with the G2 group. Overall, the study results suggested that the Biofield Energy Treated test formulation showed an improved cellular and humoral immune responses along with improved hematological and biochemical animal profile compared with the untreated test formulation. Consequently, it can be established that The Trivedi Effect®-Biofield Energy Healing has the significant capacity for immunomodulatory effect, which may also be useful in organ transplants, anti-aging, and stress management by improving overall health and quality of life.
Keywords: Biofield Energy Healers; Consciousness Energy Healing Treatment; The Trivedi Effect®; Herbomineral Formulation; Cardiac Biomarker; Testosterone; Inflammatory Diseases; Anti-aging
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Trivedi, M. K., Branton, A., Trivedi, D., Nayak, G., Afaganis, A. E., Bader, B. M., et al. (2017). Immunopotentiating Impact of The Trivedi Effect®-Biofield Energy Healing on Herbomineral Formulation After Oral Administration in Male Sprague Dawley Rats. American Journal of Bioscience and Bioengineering, 5(6). Retrieved December 16, 2019, from http://dx.doi.org/10.11648/j.bio.20170506.13
Abstract: A new proprietary herbomineral formulation was formulated, consisting of essential ingredients viz. herbal root extract ashwagandha and minerals (zinc, magnesium, and selenium). The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) Treatment on the herbomineral formulation in male Sprague Dawley rats. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from twenty renowned Biofield Energy Healers. The experimental parameters studies were humoral immune response (primary and secondary titre), delayed type hypersensitivity reaction, animal weight parameters, feed and water intake, histopathology, hematological and serum biochemistry. Humoral immune response showed the primary and secondary antibody titre values were significantly (p≤0.05) increased by 5.33% and 112.5%, respectively in the Biofield Energy Treated test formulation (G4) group with respect to the disease control group (G2). Delayed type hypersensitivity results showed significant increase in paw edema by 88.24% (p≤0.05) and 25% at 24 and 48 hours of duration, respectively in the Biofield Energy Treated test formulation (G4) group compared with the G2 group. Hematological studies showed a significant increase in the RBC count, hemoglobin, and packed cell volume (PCV), while the platelet count was increased by 8.40% in the Biofield Energy Treated test formulation group (G4) compared with the disease control group (G2). Biochemical analysis exhibited an increased level of calcium and phosphorus by 2.34% and 7.38%, respectively while serum uric acid was significantly decreased by 18.34% along with a slight increase in the magnesium, potassium, and sodium ions in the Biofield Energy Treated test formulation (G4) compared with the G2 group. In conclusion, the Biofield Energy Treated Test Formulation would be the powerful immunomodulatory product, which was found to be safe at the tested doses. Therefore, The Trivedi Effect®-Biofield Energy Healing based herbomineral formulation can be applied to potentiate the immune system that helps to fight against many infectious diseases. Overall, experimental data suggested that the Biofield Energy Treated test formulation can be used for autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.
Keywords: Biofield Energy Healers; The Trivedi Effect®; Immunopotentiation; Herbomineral Formulation; Acquired Immunity; Paw Volume; Biochemistry
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Trivedi, M. K., Branton, A., Trivedi, D., Nayak, G., Afaganis, A. E., Bader, B. M., et al. (2017). LC-MS, GC-MS, and NMR Spectroscopy Based Evaluation of the Energy of Consciousness Healing Treated Withania somnifera (Ashwagandha) Root Extract. Journal of Drug Design and Medicinal Chemistry, 3(2). Retrieved December 16, 2019, from http://dx.doi.org/10.11648/j.jddmc.20170302.11
Abstract: Withania somnifera root extract which contains withanolides as major active constituent and it is used traditionally for the prevention and treatment of several diseases. The objective of the current study was to investigate the impact of The Trivedi Effect® – Consciousness Energy Healing Treatment on the structural properties of the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while other part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The LC-MS analysis revealed that the retention time of the phytoconstituents remained same in the control and Biofield Energy Treated samples, whereas the peak area% i.e. the relative amount of the phytoconstituents at respective retention time was significantly altered. The peak area% at Rt of 5.6, 6.8, 6.9, 7.2, 7.9, 8.4, 8.5, 8.6, and 9.2 minutes of the treated sample were increased significantly in the range of 1.46% to 253.06% compared to the control sample. In the contrary, the peak area% of the treated sample at Rt of 6.4 and 8.2 minutes were significantly decreased by 12.72% and 17.35%, respectively with respect to the control sample. A total of 16 withanolides such as withanoside IV, coagulin Q, viscosa lactone B, dihydrowithanolide D, withanolide A, withaferin A, withanone, withanolide D, ixocarpalactone A, withanolide sulfoxide, withanolide B, etc. were proposed with their structure from the molecular mass at m/z 783, 621, 489, 473, 767, 471, 505, and 992 at retention times of 6.4, 6.8, 7.2, 7.9, 8.2, 8.4, and 9.1 minutes, respectively with the help of LC-MS, GC-MS and NMR data of both the control and Biofield Energy Treated samples. The mass peak intensities of the Biofield Energy Treated sample were significantly changed in the range of -81.36% to 1720.90% compared with the control sample at the same retention time. These findings suggest that The Trivedi Effect® – Consciousness Energy Healing Treatment could be beneficial for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of W. somnifera extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, aging, and other chronic infections.
Keywords: Ashwagandha; Biofield Energy Healers; The Trivedi Effect®; Consciousness Energy Healing Treatment; Biofield Energy Healing Treatment; LC-MS; Withanolides; GC-MS
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Trivedi, M. K., Branton, A., Trivedi, D., Nayak, G., Afaganis, A. E., Bader, B. M., et al. (2017). Evaluation of the Physico-chemical, Thermal and Behavioral Properties of Ashwagandha Root Extract: Effects of Consciousness Energy Healing Treatment. International Journal of Pharmacy and Chemistry, 3(3). Retrieved December 16, 2019, from http://dx.doi.org/10.11648/j.ijpc.20170303.13
Abstract: Withania somnifera (Ashwagandha) root extract has a broad range of pharmacological activities due to the presence of many biologically active metabolites. The current study was designed to evaluate the influence of The Trivedi Effect® – Consciousness Energy Healing Treatment (Biofield Energy Healing) on the physical, spectroscopic, thermal and behavioral properties of ashwagandha root extract using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Ashwagandha root extract was divided into two parts – one part was control without any Biofield Energy Treatment and another part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as Biofield Energy Treated sample. The PXRD analysis exhibited that both the control and treated samples were amorphous in nature. The particle size values at d10, d50, and d90 of the treated sample were increased significantly by 8.69%, 7.32%, and 6.50%, respectively compared with the control sample. Similarly, the surface area of the treated sample was significantly decreased by 12.69% compared to the control sample. The FT-IR analysis indicated no significant alteration of the force constant for the functional groups in the treated sample compared to the control sample. The UV-vis analysis revealed that the wavelength for the maximum absorbance of the control and treated samples were at 207.5 and 205.2 nm, respectively. The TGA revealed the two major thermal degradation steps and the total weight loss was decreased by 0.86% in the treated sample compared with the control sample. Consequently, the maximum thermal degradation temperature at 273.53°C and 389.39°C for two broad peaks in treated sample was increased by 0.78% and 0.31%, respectively compared to the control the sample (271.40°C and 388.48°C). The DSC analysis indicated that the evaporation temperature was significantly increased by 10.95% in the Biofield Energy Treated sample with a 5.34% reduced the latent heat of vaporization compared with the control sample. The overall thermal analysis indicated that the treated sample was thermally more stable as compared to the control sample. The Trivedi Effect® – Energy of Consciousness Healing Treatment might lead to have better powder flowability and long-term storage stability compared with the control sample. Thus, the treated ashwagandha root extract might provide better therapeutic response against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, ageing, and other chronic infections.
Keywords: Withania somnifera; The Trivedi Effect®; Biofield Energy Healing Treatment; Consciousness Energy Healing; PXRD; Particle Size; TGA; DSC
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Trivedi, M. K., Branton, A., Trivedi, D., Nayak, G., Afaganis, A. E., Bader, B. M., et al. (2017). Evaluation of the Physicochemical, Spectral, Thermal and Behavioral Properties of Sodium Selenate After the Energy of Consciousness Healing Treatment. Science Journal of Analytical Chemistry, 5(2). Retrieved December 16, 2019, from http://dx.doi.org/10.11648/j.sjac.20170502.12
Abstract: Sodium selenate was considered for the current study, which is an important nutrient used for the prevention and treatment of cancer, inflammatory diseases, diabetes, etc. The objective of the current study was to examine the impact of The Trivedi Effect® – Consciousness Energy Healing Treatment (Biofield Energy Healing treatment) on the physico-chemical, thermal, and behavioral properties of sodium selenate using PXRD, PSD, FT-IR, UV-vis, TGA, and DSC analysis. Sodium selenate sample was divided into two parts – one part was control, while another part was treated with the Biofield Energy remotely by twenty renowned Biofield Energy Healers and termed as the treated sample. The PXRD analysis showed that the crystallite size of the treated sample significantly altered in the range of -50.01% to 59.97% compared to the control sample. The average crystallite size of the treated sample was significantly increased by 7.32% compared with the control sample. The PSD analysis indicated that the particle size of the treated sample at d10, d50, and d90 values were increased by 6.84%, 8.04%, and 3.04%, respectively compared with the control sample. Thus, the surface area of the Biofield Energy Treated sample was significantly decreased by 7.41% compared with the control sample. The FT-IR spectra indicated the presence of sharp and strong absorption bands at 887 and 888 cm-1 due to the Se=O stretching in the control and treated sample, respectively. The wavelength of maximum absorbance from the UV-vis spectroscopic analysis of the Biofield Energy Treated sample (205.2 nm) was increased by 0.39% compared to the control sample (204.4 nm). The TGA analysis revealed the total weight loss of the Biofield Energy Treated sample was significantly reduced by 12.63% compared with the control sample. The DSC analysis showed that the vaporization temperature of the Biofield Energy Treated sample (95.14°C) was increased compared to the control sample (94.28°C). But, the latent heat of vaporization was decreased significantly in the treated sample by 43.67% compared to the control sample. Thus, The Trivedi Effect® – Consciousness Energy Healing Treatment might lead to generate a new polymorphic form of sodium selenate, which would have better powder flowability and long-term storage stability compared with the control sample. The Biofield Energy Treated sodium selenate would be helpful for designing better nutraceutical/pharmaceutical formulations against immunological disorders, inflammatory diseases, aging, stress, infectious diseases, Alzheimer’s disease, cancer, diabetes, heart diseases, etc.
Keywords: Sodium Selenate; The Trivedi Effect®; Consciousness Energy Healing Treatment; Biofield Energy Healers; PXRD; Particle Size; TGA; DSC
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