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Callas, K. J., Trivedi, M. K., Branton, A., Trivedi, D., Nayak, G., Gangwar, M., et al. (2018). Increase in Bone Mass Density and Overall Bone Health Following High-Impact of Biofield Energy Treated Vitamin D3 in MG-63 Cell Line. Significances of Bioengineering & Biosciences, 2(2). Retrieved March 24, 2019, from http://dx.doi.org/10.31031/SBB.2018.02.000533
Abstract: Vitamin D3 and calcium are the main bone health supplements required for quality of life. The present study aimed to find out the role of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health parameters in vitro using MG-63 cells. The parameters of bone health tested are Alkaline Phosphatise Enzyme (ALP) activity, collagen levels and bone mineralization. The Test Items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Krista Joanne Callas and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay exhibited increased cell viability range from 72% to 122% with safe and nontoxic profile among test samples on MG-63 cell line. ALP was significantly increased by 129.6%, 392% and 255.7% in the UT-DMEM+BT-TI, BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively at 100µg/mL as compared with the untreated group.
The level of collagen was significantly increased by 35.9% and 235.3% at 10 and 50µg/mL, respectively in the UT-DMEM+BT-TI group, while 81.7% and 197.6% at 50 and 100µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased collagen level by 184.2% and 82.3% at 50 and 100µg/mL, respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 198.6% and 53.5% at 1 and 10µg/mL, respectively in the UT-DMEM+BT-TI group, while 267.1% at 1µg/mL in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 146.2%, 91.4%, and 66.8% at 0.1,1 and 10µg/mL, respectively as compared with the untreated group. Thus, the study results concluded that Biofield Energy Treatment would be the best alternative treatment for the maintenance of strong and healthy bones and quality of life. Further, it regulates the osteoblast function and improved the level of collagen, ALP, and calcium absorption in wide range of bone dis-ordersalong with wide range of adverse bone health conditions.
Keywords: Biofield healing; Bone health; Osteosarcoma cells; Vitamin D; Bone mineralization
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Hachfeld, L. M., Trivedi, M. K., Branton, A., Trivedi, D., Nayak, G., Gangwar, M., et al. (2018). Biofield Energy Enriched Vitamin D3 versus Vitamin D3 in Preventing Fractures and Bone Loss Using MG-63 Cells. Orthopedic Research Online Journal, 3(5). Retrieved March 24, 2019, from http://dx.doi.org/10.31031/OPROJ.2018.03.000572
Abstract: Bone is a dynamic tissue, which continually adapting its structure during the development process. The aim of the study was to investigate the effect of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization using MG-63 cells. The test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Lorraine Marie Hachfeld and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability was more than 79% with a safe and nontoxic profile on MG-63 cell line. The level of ALP was increased by 400%, 84%, and 179.4% at 0.1, 1, and 10µg/mL, respectively in the UT-DMEM+BT-TI, while 314.3% at 10µg/mL in the BT-DMEM+UT-TI group as compared with the untreated test item and DMEM group. BT-DMEM+BT-TI group showed an increased ALP level by 128% and 60.3% at 1 and 10µg/mL, respectively. Collagen was significantly increased by 180% and 128.1% at 0.1 and 1µg/mL, respectively in the UT-DMEM+BT-TI group, while 84.4% and 69.7% at 1 and 10µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. Furthermore, BT-DMEM+BT-TI group showed a significant increased collagen level by 123.4% and 184.8% at 1 and 10 µg/mL, respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 385.1% at 0.1µg/mL in the UT-DMEM+BT-TI group, while 53.2%, 30.2%, and 163.9% at 0.1, 1, and 10µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 283%, 65.1%, and 197.3% at 0.1, 1, and 10µg/mL, respectively as compared with the untreated group. The experimental data suggested that the Biofield Energy Treated vitamin D3 and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. It regulates the osteoblast function, improves the bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.
Keywords: Biofield healing; Bone mass; Osteosarcoma cells; Calcium absorption; Vitamin D; Bone mineralization
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Callas, K. J., Trivedi, M. K., Branton, A., Trivedi, D., Nayak, G., Gangwar, M., et al. (2018). Increase in Bone Mass Density and Overall Bone Health Following High-Impact of Biofield Energy Treated Vitamin D3 in MG-63 Cell Line. Significances of Bioengineering & Biosciences, 2(2). Retrieved March 24, 2019, from http://dx.doi.org/10.31031/SBB.2018.02.000533
Abstract: Vitamin D3 and calcium are the main bone health supplements required for quality of life. The present study aimed to find out the role of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health parameters in vitro using MG-63 cells. The parameters of bone health tested are Alkaline Phosphatise Enzyme (ALP) activity, collagen levels and bone mineralization. The Test Items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Krista Joanne Callas and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay exhibited increased cell viability range from 72% to 122% with safe and nontoxic profile among test samples on MG-63 cell line. ALP was significantly increased by 129.6%, 392% and 255.7% in the UT-DMEM+BT-TI, BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively at 100µg/mL as compared with the untreated group.
The level of collagen was significantly increased by 35.9% and 235.3% at 10 and 50µg/mL, respectively in the UT-DMEM+BT-TI group, while 81.7% and 197.6% at 50 and 100µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased collagen level by 184.2% and 82.3% at 50 and 100µg/mL, respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 198.6% and 53.5% at 1 and 10µg/mL, respectively in the UT-DMEM+BT-TI group, while 267.1% at 1µg/mL in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 146.2%, 91.4%, and 66.8% at 0.1,1 and 10µg/mL, respectively as compared with the untreated group. Thus, the study results concluded that Biofield Energy Treatment would be the best alternative treatment for the maintenance of strong and healthy bones and quality of life. Further, it regulates the osteoblast function and improved the level of collagen, ALP, and calcium absorption in wide range of bone dis-ordersalong with wide range of adverse bone health conditions.
Keywords: Biofield healing; Bone health; Osteosarcoma cells; Vitamin D; Bone mineralization
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Hachfeld, L. M., Trivedi, M. K., Branton, A., Trivedi, D., Nayak, G., Gangwar, M., et al. (2018). Biofield Energy Enriched Vitamin D3 versus Vitamin D3 in Preventing Fractures and Bone Loss Using MG-63 Cells. Orthopedic Research Online Journal, 3(5). Retrieved March 24, 2019, from http://dx.doi.org/10.31031/OPROJ.2018.03.000572
Abstract: Bone is a dynamic tissue, which continually adapting its structure during the development process. The aim of the study was to investigate the effect of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization using MG-63 cells. The test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Lorraine Marie Hachfeld and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability was more than 79% with a safe and nontoxic profile on MG-63 cell line. The level of ALP was increased by 400%, 84%, and 179.4% at 0.1, 1, and 10µg/mL, respectively in the UT-DMEM+BT-TI, while 314.3% at 10µg/mL in the BT-DMEM+UT-TI group as compared with the untreated test item and DMEM group. BT-DMEM+BT-TI group showed an increased ALP level by 128% and 60.3% at 1 and 10µg/mL, respectively. Collagen was significantly increased by 180% and 128.1% at 0.1 and 1µg/mL, respectively in the UT-DMEM+BT-TI group, while 84.4% and 69.7% at 1 and 10µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. Furthermore, BT-DMEM+BT-TI group showed a significant increased collagen level by 123.4% and 184.8% at 1 and 10 µg/mL, respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 385.1% at 0.1µg/mL in the UT-DMEM+BT-TI group, while 53.2%, 30.2%, and 163.9% at 0.1, 1, and 10µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 283%, 65.1%, and 197.3% at 0.1, 1, and 10µg/mL, respectively as compared with the untreated group. The experimental data suggested that the Biofield Energy Treated vitamin D3 and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. It regulates the osteoblast function, improves the bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.
Keywords: Biofield healing; Bone mass; Osteosarcoma cells; Calcium absorption; Vitamin D; Bone mineralization
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Callas, K. J., Trivedi, M. K., Branton, A., Trivedi, D., Nayak, G., Gangwar, M., et al. (2018). Increase in Bone Mass Density and Overall Bone Health Following High-Impact of Biofield Energy Treated Vitamin D3 in MG-63 Cell Line. Significances of Bioengineering & Biosciences, 2(2). Retrieved March 24, 2019, from http://dx.doi.org/10.31031/SBB.2018.02.000533
Abstract: Vitamin D3 and calcium are the main bone health supplements required for quality of life. The present study aimed to find out the role of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health parameters in vitro using MG-63 cells. The parameters of bone health tested are Alkaline Phosphatise Enzyme (ALP) activity, collagen levels and bone mineralization. The Test Items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Krista Joanne Callas and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay exhibited increased cell viability range from 72% to 122% with safe and nontoxic profile among test samples on MG-63 cell line. ALP was significantly increased by 129.6%, 392% and 255.7% in the UT-DMEM+BT-TI, BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively at 100µg/mL as compared with the untreated group.
The level of collagen was significantly increased by 35.9% and 235.3% at 10 and 50µg/mL, respectively in the UT-DMEM+BT-TI group, while 81.7% and 197.6% at 50 and 100µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased collagen level by 184.2% and 82.3% at 50 and 100µg/mL, respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 198.6% and 53.5% at 1 and 10µg/mL, respectively in the UT-DMEM+BT-TI group, while 267.1% at 1µg/mL in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 146.2%, 91.4%, and 66.8% at 0.1,1 and 10µg/mL, respectively as compared with the untreated group. Thus, the study results concluded that Biofield Energy Treatment would be the best alternative treatment for the maintenance of strong and healthy bones and quality of life. Further, it regulates the osteoblast function and improved the level of collagen, ALP, and calcium absorption in wide range of bone dis-ordersalong with wide range of adverse bone health conditions.
Keywords: Biofield healing; Bone health; Osteosarcoma cells; Vitamin D; Bone mineralization
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