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Author (up) Alice Branton url  doi
openurl 
  Title Antibiogram and Genotypic Analysis using 16S rDNA after Biofield Treatment on Morganella morganii Type Journal Article
  Year 2015 Publication Advanced Techniques in Biology & Medicine Abbreviated Journal  
  Volume 3 Issue 3 Pages  
  Keywords Morganella Morganii; Antimicrobial Susceptibility; Biochemical Reaction; Biotype; 16s Rdna Analysis; Gene Sequencing Of 16s Rdna; 16s Rdna Amplification; Β-Lactam Antibiotics; Β-Lactamase Enzymes; Antimicrobials; Morganella Strain; Catheter-Associated Bacteriuria; Polymerase Chain Reaction (pcr); Trivedi Effect; The Trivedi Effect; Mahendra Kumar Trivedi; Mahendra Trivedi; Biofield; Biofield Treatment  
  Abstract Morganella morganii (M. morganii) is one of the important nosocomial pathogen associated with the urinary tract infections and bacteremia. The aim of this study was to evaluate the effect of Mr. Trivedi’s biofield energy treatment on M. morganii in the lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, biotype number and genotype. M. morganii cells were procured from MicroBioLogics Inc., USA in sealed packs bearing the American Type Culture Collection (ATCC 25829) number and stored according to the recommended storage protocols until needed for experiments. M. morganii strain was divided into two groups, Group (Gr.) I: control and Gr. II: treated. Gr. II was further subdivided into two groups, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzed on day 142 (Study I). After retreatment on day 142, the sample (Study II) was divided into three separate tubes. First, second and third tube was further analyzed on day 5, 10 and 15 respectively. All experimental parameters were studied using the automated MicroScan Walk-Away® system. The 16S rDNA sequencing of lyophilized treated sample was carried out to correlate the phylogenetic relationship of M. morganii with other bacterial species. Antimicrobial susceptibility results showed 32.14% alterations, while minimum inhibitory concentration results showed 18.75% alterations of the tested antimicrobials. Biochemical study also showed altered positive reactions in nitrofurantoin and indole with respect to control. Biotype study showed alteration in Gr. IIB, study II, on day 15 (4005 1446) as compared to the control (4004 1446). 16S rDNA sequencing analysis showed similar results with the identified microbe as M. morganii (GenBank accession number: AB210972) having 80% identity of the gene sequencing data. Total 1507 base nucleotide of 16S rDNA gene sequences were analyzed by multiple alignments, while nearest homolog genus-species of M. morganii was found as Providencia rettgeri (accession number: AM040492). These results suggested that biofield treatment has a significant impact on M. morganii in lyophilized as well as revived state.  
  Address  
  Corporate Author Thesis  
  Publisher Omics Group Place of Publication USA Editor  
  Language English Summary Language English Original Title Antibiogram and Genotypic Analysis using 16S rDNA after Biofield Treatment on Morganella morganii  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 2379-1764 ISBN Medium  
  Area Microbiology Expedition Conference  
  Notes Approved yes  
  Call Number Trivedi Global Inc. @ alice @ Serial 43156  
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