||Withania somnifera (Ashwagandha) root extract and their active metabolites possess a broad range of pharmacological activities. The current study aimed to explore the impact of The Trivedi Effect® – Energy of Consciousness Healing Treatment (Biofield Energy Healing) on the physical, spectroscopic, and thermal properties of ashwagandha root extract using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Ashwagandha root extract was divided into two parts – one part was control without any and another part was treated with The Trivedi Effect® – Energy of Consciousness Healing Treatment remotely by eighteen renowned Biofield Energy Healers and defined as The Trivedi Effect® treated sample. The PXRD analysis exhibited that both the treated and control samples were amorphous in nature. The particle sizes at d10, d50, and d90 values of the treated sample were significantly decreased by 12.23%, 10.97%, and 1.25%, respectively with respect to the control sample. The surface area analysis revealed that the surface area of the treated sample was significantly increased by 14.29% as compared to the control sample. The FT-IR analysis indicated the alteration of the force constant for the functional groups of the treated sample in comparison to the control sample. The UV-vis analysis revealed that the wavelength for the maximum absorbance of the control and treated samples were at 205.8 and 206.0 nm, respectively. The TGA analysis revealed that the total weight loss was decreased by 0.70% in the treated sample as compared to the control sample. This indicated that the treated sample was thermally more stable as compared to the control sample. The DSC analysis indicated that the melting point of the treated sample (190.48°C) was decreased by 4.91% than the control sample (203.45°C). The latent heat of fusion of the treated (4.94 J/g) sample was significantly decreased by 42.96% compared with the control (8.66 J/g) sample. The current findings suggest that The Trivedi Effect® – Energy of Consciousness Healing Treatment (Biofield Energy Healing) might have the astounding capacity to enhance the solubility, absorption, dissolution, and finally, bioavailability of ashwagandha root extract in various form of pharmaceutical and nutraceutical formulation by modifying its particle size and surface area. Thus, the treated ashwagandha root extract might provide better therapeutic response against against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, aging and other chronic infections.