||There are numerous scientific studies on vitamin D3 reported to have many important roles viz. in bone metabolism, osteoporosis, immunity, and useful in several diseases, e.g., cancers, cardio-vascular, and neurodegenerative diseases. Surprisingly, there is little information available about the concentration of 25-hydroxyvitamin D3 in the blood, which is the best indicator of vitamin D3 status after its oral absorption. However, the biological activity of vit-D3 is mediated via the formation of the active metabolite, 1-α, 25-dihydroxyvitamin D3. There are several factors which affect its absorption and first-pass metabolism that lead to have very low plasma concentrations of both the metabolites (25-hydroxyvitamin D3 and 1-α, 25-dihydroxyvitamin D3) following an oral administration. Therefore, the present study was performed to determine the effects of The Trivedi Effect®-Energy of Consciousness Healing Treatment on vit-D3 and rats through the measurement of plasma 25-hydroxyvitamin D3 concentrations after the oral administration of vit-D3 in rats. The test item, vit-D3 was divided into two parts. One part was denoted as the control (without Biofield Energy Healing Treatment), while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment by renowned Biofield Energy Healer, Alice Branton. Additionally, one group of animals also received Consciousness Energy Healing Treatment per se by the Healer under similar conditions. Vitamin D3 oral formulations were administrated by oral gavage at a dose of 500 µg per kg in groups viz. G1 (untreated Vitamin D3), G2 (Biofield Treated Vitamin D3) and G3 (Biofield Treated animals received untreated Vitamin D3) group. The Biofield Energy Healing Treatment significantly altered the relative oral exposure (AUC0–t) of 25-hydroxyvitamin D3 by 54.62% in G2 group compared to the control group, G1. The Biofield Energy Treatment also altered plasma peak concentration (Cmax) of 25-hydroxyvitamin D3 by 53.8% and 7.6% in G2 and G3 groups, respectively compared to the control group. After oral administration, plasma concentrations of 25-hydroxyvitamin D3 in control group (G1) declined with slow excretion rate and the elimination half-life (T1/2) was 100.59 hours. The oral elimination half-life (T1/2) of 25-hydroxyvitamin D3 in G2 and G3 were 69.09 hours and 55.76 hours, respectively. The mean residence time (MRTlast) and elimination rate constant (Kel) remained unaltered in all three groups. The Trivedi Effect®-Energy of Consciousness Healing Treatment could be an innovative strategy which opens new avenues to overcome poorly absorbed pharmaceuticals/nutraceuticals/herbal extracts and can improve the therapeutic performance of orally active molecules.