||The study was initiated to investigate the effect of Biofield Treatment on DMEM medium for bone health in MG-63 cells. The test item, DMEM medium was divided into two parts. The test item received Biofield Treatment by a renowned Biofield Energy Healer, Dahryn Trivedi and was labeled as the Biofield Treated DMEM, while the other part did not receive any treatment and denoted as untreated DMEM group. MTT assay showed that cell viability of the test item was more than 77%, which indicate a safe and non-toxic profile of the test items in MG-63 cells. The level of ALP was significantly (p≤0.001) increased by 42.86% in Biofield Treated DMEM compared with untreated DMEM. Overall, data envisaged that the Biofield Treated DMEM would play an important role for the growth of MG-63 cells along with an improved bone health parameter i.e. ALP level, which plays vital role in the promotion and maintenance of strong and healthy bones. Further, it could be used in numerous bone-related disorders viz. rickets, osteoporosis, deformed bones, Paget’s disease of bone, bone loss and fractures, osteomalacia, bone and/or joint pain, osteoma, hormonal imbalance, stress, and aging.