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Author (up) Crescenzi, O.; Tomaselli, S.; Guerrini, R.; Salvadori, S.; D’Ursi, A.M.; Temussi, P.A.; Picone, D. openurl 
  Title Solution structure of the Alzheimer amyloid β‐peptide (1–42) in an apolar microenvironment Type Journal Article
  Year 2002 Publication Abbreviated Journal European Journal of Biochemistry  
  Volume 269 Issue 22 Pages 5642-5648  
  Keywords  
  Abstract The major components of neuritic plaques found in Alzheimer disease (AD) are peptides known as amyloid β-peptides (Aβ), which derive from the proteolitic cleavage of the amyloid precursor proteins. In vitro Aβ may undergo a conformational transition from a soluble form to aggregated, fibrillary β-sheet structures, which seem to be neurotoxic. Alternatively, it has been suggested that an α-helical form can be involved in a process of membrane poration, which would then trigger cellular death. Conformational studies on these peptides in aqueous solution are complicated by their tendency to aggregate, and only recently NMR structures of Aβ-(1–40) and Aβ-(1–42) have been determined in aqueous trifluoroethanol or in SDS micelles. All these studies hint to the presence of two helical regions, connected through a flexible kink, but it proved difficult to determine the length and position of the helical stretches with accuracy and, most of all, to ascertain whether the kink region has a preferred conformation. In the search for a medium which could allow a more accurate structure determination, we performed an exhaustive solvent scan that showed a high propensity of Aβ-(1–42) to adopt helical conformations in aqueous solutions of fluorinated alcohols. The 3D NMR structure of Aβ-(1–42) shows two helical regions encompassing residues 8–25 and 28–38, connected by a regular type I β-turn. The surprising similarity of this structure, as well as the sequence of the C-terminal moiety, with those of the fusion domain of influenza hemagglutinin suggests a direct mechanism of neurotoxicity.  
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  Series Volume Series Issue Edition  
  ISSN 1432-1033 ISBN Medium  
  Area Expedition Conference  
  Notes Approved no  
  Call Number AG @ matthewjvarga @ Serial 47188  
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