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Author Trivedi, Mahendra Kumar; Branton, Alice; Trivedi, Dahryn; Nayak, Gopal; Gangwar, Mayank; Jana, Snehasis url  doi
openurl 
  Title Morphological and Molecular Analysis Using RAPD in Biofield Treated Sponge and Bitter Gourd Type Journal Article
  Year 2015 Publication American Journal of Agriculture and Forestry Abbreviated Journal  
  Volume 3 Issue 6 Pages 264-270  
  Keywords Luffa cylindrical; Momordica charantia; Biofield energy; Growth Attributes; DNA Fingerprinting; Polymorphism  
  Abstract Plants are known to have sense and ca respond to touch, electric and magnetic field. The present study was designed on the sponge gourd (Luffa cylindrica) and bitter gourd (Momordica charantia) seeds with respect to biofield energy treatment. The seeds of each crop were divided into two groups, one was kept control, while the other group was subjected to Mr. Trivedi’ biofield energy treatment. The variabilities in growth contributing parameters were studied and compared with their control. To study the genetic variability after biofield energy treatment, both the seeds were analyzed for DNA fingerprinting using RAPD method. After germination, the plants of sponge gourd were reported to have uniform colored leaves and strong stem. The leaves and fruits of sponge gourd showed no infection, with anti-gravity properties during early stage of fruiting as compared with the control group. Similarly, treated bitter gourd showed uniform color of leaves, strong stem, with disease free fruits in biofield treated seeds as compared with the control. The true polymorphism (%) observed between control and treated samples of sponge and bottle gourd seed sample was an average value of 7.8% and 66% respectively. In conclusion, Mr. Trivedi’s biofield energy treatment has the ability to alter the plant growth rate that may by interacting with plant genome, which resulted in high yield of crops.  
  Address  
  Corporate Author Thesis  
  Publisher Science Publishing Group Place of Publication United States Editor  
  Language English Summary Language English Original Title Morphological and Molecular Analysis Using RAPD in Biofield Treated Sponge and Bitter Gourd  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 2330-8583 ISBN Medium  
  Area Agriculture Expedition Conference  
  Notes Approved (down) yes  
  Call Number Trivedi Global Inc. @ mahendra @ Serial 42700  
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Author Trivedi, Mahendra Kumar; Branton, Alice; Trivedi, Dahryn; Nayak, Gopal; Saikia, Gunin; Jana, Snehasis url  doi
openurl 
  Title Mass Spectrometry Analysis of Isotopic Abundance of 13C, 2H, or 15N in Biofield Energy Treated Aminopyridine Derivatives Type Journal Article
  Year 2015 Publication American Journal of Physical Chemistry Abbreviated Journal  
  Volume 4 Issue 6 Pages 65-70  
  Keywords Biofield Energy Treatment; 2-Aminopyridine; 2;6-Diaminopyridine; Gas Chromatography-Mass Spectrometry  
  Abstract 2-Aminopyridine (2-AP) and 2,6-diaminopyridine (2,6-DAP) are two derivatives of aminopyridines that act as an important organic intermediates, mostly used in medicines, dyes and organic sensors. The aim of the study was to evaluate the impact of biofield energy treatment on isotopic abundance ratios of 2H/1H, 13C/12C, or 15N/14N, in aminopyridine derivatives using gas chromatography-mass spectrometry (GC-MS). The 2-AP and 2,6-DAP samples were divided into two parts: control and treated. The control sample remained as untreated, while the treated sample was further divided into four groups as T1, T2, T3, and T4. The treated group was subjected to Mr. Trivedi’s biofield energy treatment. The GC-MS spectra of 2-AP and 2,6- DAP showed five and six m/z peaks respectively due to the molecular ion peak and fragmented peaks of aminopyridine derivatives. The isotopic abundance ratio of 2H/1H, 13C/12C, or 15N/14N were calculated for both the derivatives and significant alteration was found in the treated samples as compared to the respective control. The isotopic abundance ratio of 2H/1H, 13C/12C, or 15N/14N in treated samples of 2-AP was decreased by 55.83% in T1 and significantly increased by 202.26% in T4. However, in case of 2,6-DAP, the isotopic abundance ratio of 2H/1H, 13C/12C, and 15N/14N, in the treated sample showed a significant increase (up to 370.54% in T3) with respect to the control. GC-MS data suggested that the biofield energy treatment on aminopyridine derivatives had significantly altered the isotopic abundance of 2H, 13C, or 15N in the treated 2-AP and 2,6- DAP as compared to the control.  
  Address  
  Corporate Author Thesis  
  Publisher Science PG Place of Publication United States Editor  
  Language English Summary Language English Original Title Mass Spectrometry Analysis of Isotopic Abundance of 13 C, 2 H, or 15 N in Biofield Energy Treated Aminopyridine Derivatives  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 2327-2430 ISBN Medium  
  Area Pharmaceuticals Expedition Conference  
  Notes Approved (down) yes  
  Call Number Trivedi Global Inc. @ mahendra @ Serial 42701  
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Author Mahendra Kumar Trivedi url  doi
openurl 
  Title Bio-field Treatment: An Effective Strategy to Improve the Quality of Beef Extract and Meat Infusion Powder Type Journal Article
  Year 2015 Publication Journal of Nutrition and Food Sciences Abbreviated Journal  
  Volume 5 Issue 4 Pages  
  Keywords Beef extract powder; meat infusion powder; beef powder characteristics; beef extract xrd analysis; quality of beef extract; Mahendra Kumar Trivedi; Mahendra Trivedi; Trivedi Effect; The Trivedi Effect; Biofield; Biofield Energy Treatment Beef extract powder; Meat infusion powder; Bio-field treatment; Fourier transform infrared spectroscopy X-ray diffraction; Particle size analysis; Surface area analysis; Differential scanning calorimetry; Thermo gravimetric analysis.  
  Abstract The present research work investigated the influence of bio-field treatment on two common flavoring agents used in food industries namely beef extract powder (BEP) and meat infusion powder (MIP). The treated powders were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), particle size analysis,surface area analysis, differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). The FT-IR results showed disappearance of triglycerides peaks in both the treated powders as compared to control. XRD results corroborated the amorphous nature of both control and treated samples. The BEP showed enhanced average particle size (d50) and d99(size exhibited by 99% of powder particles) by 5.7% and 16.1%, respectively as compared to control. Contrarily, the MIP showed a decreased particle size (d50;0.4% and d99; 18.1%) as compared to control.It was assumed that enormous energy was stored in MIP after bio-field treatment that led to fracture into smaller particles. The surface area was increased in both the treated powders. DSC result showed significant increase in melting temperature, in BEP and MIP, which indicated the higher thermal stability of the samples. However, the specific heat capacity (∆H) was decreased in both samples, which was probably due to high energy state of the powders.  
  Address  
  Corporate Author Thesis  
  Publisher Nutrition and Food Sciences Place of Publication United States Editor  
  Language English Summary Language English Original Title Bio-field Treatment: An Effective Strategy to Improve the Quality of Beef Extract and Meat Infusion Powder  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume 5 Series Issue 4 Edition  
  ISSN 2155-9600 ISBN Medium  
  Area Pharmaceuticals Expedition Conference  
  Notes Approved (down) yes  
  Call Number Trivedi Global Inc. @ mahendra @ Serial 42702  
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Author Trivedi, Mahendra Kumar; Nayak, Gopal; Patil, Shrikant; Tallapragada, Rama Mohan; Jana, Snehasis; Mishra, Rakesh Kumar url  doi
openurl 
  Title Evaluation of the Impact of Biofield Treatment on Physical and Thermal Properties of Casein Enzyme Hydrolysate and Casein Yeast Peptone Type Journal Article
  Year 2015 Publication Clinical Pharmacology & Biopharmaceutics Abbreviated Journal  
  Volume 4 Issue 2 Pages  
  Keywords Casein enzyme hydrolysate; Casein yeast peptone; Biofield treatment; FT-IR; TGA; DSC; Particle size and Surface area  
  Abstract In the present study, the influence of biofield treatment on physical and thermal properties of Casein Enzyme Hydrolysate (CEH) and Casein Yeast Peptone (CYP) were investigated. The control and treated samples were characterized by Fourier transform infrared (FT-IR) spectroscopy, differential scanning calorimetry (DSC), Thermo Gravimetric Analysis (TGA), particle size and surface area analysis. The FTIR results revealed that biofield treatment has caused reduction of amide group (amide-I and amide-II) stretching vibration peak that is associated with strong intermolecular hydrogen bonding in treated CEH as compared to control. However, no significant changes were observed in FTIR spectrum of treated CYP. The TGA analysis of treated CEH showed a substantial improvement in thermal stability which was confirmed by increase in maximum thermal decomposition temperature (217°C) as compared to control (209°C). Similarly, the treated CYP also showed enhanced thermal stability as compared to control. DSC showed increase in melting temperature of treated CYP as compared to control. However the melting peak was absent in DSC of treated CEH which was probably due to rigid chain of the protein. The surface area of treated CEH was increased by 83% as compared to control. However, a decrease (7.3%) in surface area was observed in treated CYP. The particle size analysis of treated CEH showed a significant increase in average particle size (d50) and d99 value (maximum particle size below which 99% of particles are present) as compared to control sample. Similarly, the treated CYP also showed a substantial increase in d50 and d99 values which was probably due to the agglomeration of the particles which led to formation of bigger microparticles. The result showed that the biofield treated CEH and CYP could be used as a matrix for pharmaceutical applications.  
  Address  
  Corporate Author Thesis  
  Publisher OMICS Publishing Group Place of Publication United States Editor  
  Language English Summary Language English Original Title Evaluation of the Impact of Biofield Treatment on Physical and Thermal Properties of Casein Enzyme Hydrolysate and Casein Yeast Peptone  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 2167-065X ISBN Medium  
  Area Pharmaceuticals Expedition Conference  
  Notes Approved (down) yes  
  Call Number Trivedi Global Inc. @ mahendra @ Serial 42703  
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Author Trivedi, Mahendra Kumar; Branton, Alice; Trivedi, Dahryn; Nayak, Gopal; Saikia, Gunin; Jana, Snehasis url  doi
openurl 
  Title Isotopic Abundance Analysis of Biofield Treated Benzene, Toluene and p-Xylene Using Gas Chromatography-Mass Spectrometry (GC-MS) Type Journal Article
  Year 2015 Publication Mass Spectrometry & Purification Techniques Abbreviated Journal  
  Volume 1 Issue 1 Pages  
  Keywords Biofield treatment; Benzene; Gas Chromatography-Mass Spectrometry; Toluene; p-xylene  
  Abstract Benzene, toluene and p-xylene are derivatives of benzene, generally produced from crude petroleum and have numerous applications in industry. The aim of the present study was to evaluate the impact of biofield treatment on isotopic abundance of these benzene derivatives by gas chromatography-mass spectrometry (GC-MS). Benzene, toluene and p-xylene samples were divided into two parts: control and treatment. Control part was remained as untreated and treatment part was subjected to Mr. Trivedi’s biofield treatment. Control and treated samples were characterized using GC-MS. GC-MS data revealed that isotopic abundance ratio of 13C12C or 2H/1H (PM+1/PM) of treated samples were significantly increased from un-substituted to substituted benzene rings (where, PM- primary molecule, PM+1- isotopic molecule either for 13C/12C and/or 2H/1H). The isotopic abundance ratio of 13C/12C or 2H/1H (PM+1/PM) in benzene was decreased significantly by 42.14% as compared to control. However, the isotopic abundance ratio of (PM+1/PM) in treated toluene and p-xylene was significantly increased up to 531.61% and 134.34% respectively as compared to their respective control. Thus, overall data suggest that biofield treatment has significantly altered the isotopic abundance ratio of (PM+1/PM) in a different way for un-substituted and substituted benzenes.  
  Address  
  Corporate Author Thesis  
  Publisher OMICS Publishing Group Place of Publication United States Editor  
  Language English Summary Language English Original Title Isotopic Abundance Analysis of Biofield Treated Benzene, Toluene and p-Xylene Using Gas Chromatography-Mass Spectrometry (GC-MS)  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 2469-9861 ISBN Medium  
  Area Pharmaceuticals Expedition Conference  
  Notes Approved (down) yes  
  Call Number Trivedi Global Inc. @ mahendra @ Serial 42704  
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Author Trivedi, Mahendra Kumar; Branton, Alice; Trivedi, Dahryn; Nayak, Gopal; Singh, Ragini; Jana, Snehasis url  doi
openurl 
  Title Evaluation of Physical, Thermal and Spectroscopic Properties of Biofield Treated p-Hydroxyacetophenone Type Journal Article
  Year 2015 Publication Natural Products Chemistry & Research Abbreviated Journal  
  Volume 3 Issue 5 Pages  
  Keywords Biofield energy treatment; p-Hydroxyacetophenone; X-ray diffraction; Surface area analysis; Thermogravimetric analysis; Ultraviolet-visible spectroscopy; Fourier transform infrared spectroscopy  
  Abstract P-Hydroxyacetophenone (pHAP) is an aromatic ketone derivative that is mainly used in the manufacturing of various pharmaceuticals, flavours, fragrances, etc. In the present study, the impact of Mr. Trivedi’s biofield energy treatment was analysed on various properties of pHAP viz. crystallite size, surface area, melting temperature, thermal decomposition, and spectral properties. The pHAP sample was divided into two parts; one was kept as control sample while another part was named as treated sample. The treated sample was given the biofield energy treatment and various parameters were analysed as compared to the control sample by X-ray diffraction (XRD), surface area analyser, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), ultravioletvisible (UV-VIS), and Fourier transform infrared (FT-IR) spectroscopy. The XRD studies showed the decrease in crystallite size of the treated sample (61.25 nm) as compared to the control (84.18 nm); however the intensity of peaks in diffractogram was increased in treated sample. Besides, the surface area of treated sample was decreased by 41.17% as compared to the control. The TGA analysis revealed that onset temperature as well as Tmax (maximum thermal decomposition temperature) was increased in the treated sample. However, the latent heat of fusion (ΔH) was decreased from 124.56 J/g (control) to 103.24 J/g in the treated sample. The treated and control samples were also evaluated by UV-Vis and FT-IR spectroscopy and did not show any significant alteration in spectra of treated sample as compared to the respective control. Hence, the overall results suggest that there was an impact of biofield energy treatment on the physical and thermal properties of pHAP sample.  
  Address  
  Corporate Author Thesis  
  Publisher Esciencecentral organisation Place of Publication United States Editor  
  Language English Summary Language English Original Title Evaluation of Physical, Thermal and Spectroscopic Properties of Biofield Treated p-Hydroxyacetophenone  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 2329-6836 ISBN Medium  
  Area Pharmaceuticals Expedition Conference  
  Notes Approved (down) yes  
  Call Number Trivedi Global Inc. @ mahendra @ Serial 42705  
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Author Trivedi, Mahendra Kumar; Branton, Alice; Trivedi, Dahryn; Nayak, Gopal; Bairwa, Khemraj; Jana, Snehasis url  doi
openurl 
  Title Physicochemical and Spectroscopic Characterization of Biofield Energy Treated p-Anisidine Type Journal Article
  Year 2015 Publication Pharmaceutical Analytical Chemistry: Open Access Abbreviated Journal  
  Volume 1 Issue 1 Pages  
  Keywords p-Anisidine; X-ray diffraction; Surface area analysis; Differential scanning calorimetry; Fourier transform infrared; Biofield energy  
  Abstract The p-anisidine is widely used as chemical intermediate in the production of various dyes, pigments, and pharmaceuticals. This study was aimed to evaluate the effect of biofield energy treatment on the physicochemical and spectroscopic properties of p-anisidine. The study was performed after dividing the sample in two groups; one was remained as untreated and another was subjected to Mr. Trivedi’s biofield energy treatment. Afterward, both the control and treated samples of p-anisidine were evaluated using X-ray diffraction (XRD), surface area analyzer, differential scanning calorimetry (DSC), thermogravimetric analysis-derivative thermogravimetry (TGA-DTG), Fourier transform infrared (FT-IR), and ultraviolet-visible (UV-Vis) spectroscopy. The XRD analysis showed the increase in unit cell volume from 683.81 → 690.18 × 10-24 cm3 and crystallite size from 83.84→84.62 nm in the treated sample with respect to the control. The surface area analysis exhibited the significant increase (25.44%) in the surface area of treated sample as compared to control. The DSC thermogram of control p-anisidine showed the latent heat of fusion and melting temperature and 146.78 J/g and 59.41°C, respectively, which were slightly increased to 148.89 J/g and 59.49°C, respectively after biofield treatment. The TGA analysis showed the onset temperature of thermal degradation at 134.68°C in the control sample that was increased to 150.02°C after biofield treatment. The result showed about 11.39% increase in onset temperature of thermal degradation of treated p-anisidine as compared to the control. Moreover, the Tmax (temperature at which maximum thermal degradation occurs) was also increased slightly from 165.99°C (control) to 168.10°C (treated). This indicated the high thermal stability of treated p-anisidine as compared to the control. However, the FT-IR and UV spectroscopic studies did not show any significant changes in the spectral properties of treated p-anisidine with respect to the control.All together, the XRD, surface area and thermal analysis suggest that Mr. Trivedi’s biofield energy treatment has the impact on physical and thermal properties of the treated p-anisidine.  
  Address  
  Corporate Author Thesis  
  Publisher OMICS Publishing Group Place of Publication United States Editor  
  Language English Summary Language English Original Title Physicochemical and Spectroscopic Characterization of Biofield Energy Treated p-Anisidine  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 2471-2698 ISBN Medium  
  Area Pharmaceuticals Expedition Conference  
  Notes Approved (down) yes  
  Call Number Trivedi Global Inc. @ mahendra @ Serial 42706  
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Author Trivedi, Mahendra Kumar; Branton, Alice; Trivedi, Dahryn; Gangwar, Mayank; Jana, Snehasis url  doi
openurl 
  Title Antimicrobial Susceptibility, Biochemical Characterization and Molecular Typing of Biofield Treated Klebsiella pneumoniae Type Journal Article
  Year 2015 Publication Journal of Health & Medical Informatics Abbreviated Journal  
  Volume 6 Issue 5 Pages  
  Keywords Klebsiella pneumoniae; Biofield energy treatment; Antibiogram; Biochemical reactions; Polymorphism; Random Amplified Polymorphic DNA  
  Abstract Pathogenic isolates of Klebsiella pneumoniae (K. pneumoniae), particularly the extended-spectrum β-lactamase (ESBL) producing strains, are mostly associated with the failure of antibiotic therapy in nosocomial infections. The present work was designed to evaluate the impact of Mr. Trivedi’s biofield energy treatment on phenotypic and genotypic characteristics of K. pneumoniae. The strain of K. pneumoniae bearing ATCC 15380 (American Type Culture Collection) was procured from the Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to Mr. Trivedi’s biofield energy treatment and analyzed for the antimicrobial susceptibility, minimum inhibitory concentration (MIC), biochemical reactions, and biotyping using automated MicroScan Walk-Away® system. Further, the effect of biofield treatment was also evaluated using Random Amplified Polymorphic DNA (RAPD) in order to determine their epidemiological relatedness and genetic characteristics of biofield treated K. pneumoniae samples. The antimicrobial susceptibility results showed an improve sensitivity (i.e. from intermediate to susceptible) of ampicillin/sulbactam and chloramphenicol, while altered sensitivity of cephalothin (i.e. from susceptible to intermediate) was also reported as compared to the control sample. The MIC value showed two-fold decrease in MIC value of ampicillin/sulbactam (i.e. 16/8 to ≤8/4 μg/mL) and chloramphenicol (i.e. 16 to ≤ 8 μg/mL) as compared to the control. The cephalothin showed two-folds change (i.e. ≤ 8 to 16 μg/mL) in the MIC value as compared with the control. Biofield treatment showed 9.09% alterations in biochemical reactions followed by a change in biotype number (7774 4272) in the treated group with respect to the control (7774 4274). Genetic fingerprinting was performed on control and treated samples using RAPD-PCR biomarkers, which showed an average range of 11 to 15% of polymorphism among the treated samples with respect to the control. These results suggested that Mr. Trivedi’s biofield energy treatment has a significant impact on K. pneumoniae.  
  Address  
  Corporate Author Thesis  
  Publisher OMICS Publishing Group Place of Publication United States Editor  
  Language English Summary Language English Original Title Antimicrobial Susceptibility, Biochemical Characterization and Molecular Typing of Biofield Treated Klebsiella pneumoniae  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 2157-7420 ISBN Medium  
  Area Microbiology Expedition Conference  
  Notes Approved (down) yes  
  Call Number Trivedi Global Inc. @ mahendra @ Serial 42707  
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Author Trivedi, Mahendra Kumar; Branton, Alice; Trivedi, Dahryn; Shettigar, Harish; Nayak, Gopal; Gangwar, Mayank; Jana, Snehasis url  doi
openurl 
  Title Assessment of Antibiogram of Multidrug-Resistant Isolates of Enterobacter aerogenes after Biofield Energy Treatment Type Journal Article
  Year 2015 Publication Pharmaceutical Care & Health Systems Abbreviated Journal  
  Volume 2 Issue 5 Pages  
  Keywords Enterobacter aerogenes; Multidrug resistant; Antimicrobial susceptibility; Biofield treatment; Biochemical reactions; Biotyping  
  Abstract Enterobacter aerogenes (E. aerogenes) has been reported as the versatile opportunistic pathogen associated with the hospital infections worldwide. The aim of the study was to determine the impact of Mr. Trivedi’s biofield energy treatment on multidrug resistant clinical lab isolates (LSs) of E. aerogenes. The MDR isolates of E. aerogenes (i.e., LS 45 and LS 54) were divided into two groups, i.e., control and treated. Samples were analyzed for antimicrobial susceptibility pattern, minimum inhibitory concentration (MIC), biochemical study, and biotype number using MicroScan Walk-Away® system, on day 10 after the biofield treatment. The antimicrobial sensitivity assay showed 14.28% alteration out of twenty eight tested antimicrobials with respect to the control. The cefotetan sensitivity changed from intermediate (I) to inducible β-lactamase (IB), while piperacillin/tazobactam changed from resistant to IB in the treated LS 45. Improved sensitivity was reported in tetracycline, i.e., from I to susceptible (S) in LS 45, while chloramphenicol and tetracycline sensitivity changed from R to I in treated LS 54. Four-fold decrease in MIC value was reported in piperacillin/tazobactam, and two-fold decrease in cefotetan and tetracycline in the biofield treated LS 45 as compared to the control. MIC results showed an overall decreased MIC values in 12.50% tested antimicrobials such as chloramphenicol (16 μg/mL) and tetracycline (8 μg/mL) in LS 54. The biochemical study showed an overall 45.45% negative reaction in the tested biochemical in both the treated isolates as compared to the control. A change in biotype number was reported in MDR isolates (LS 45 and LS 54), while in LS 54, altered biotype number, i.e., 0406 0374 as compared to the control (7770 4376), with identification of the new species as Stenotrophomonas maltophilia with brown color as special characteristic. The study findings suggest that Mr. Trivedi’s biofield energy treatment on clinical MDR isolates of E. aerogenes has the significant effect on altering the sensitivity of antimicrobials, decreasing the MIC values, changed biochemical reactions, and biotype number.  
  Address  
  Corporate Author Thesis  
  Publisher OMICS Publishing Group Place of Publication United States Editor  
  Language English Summary Language English Original Title Assessment of Antibiogram of Multidrug-Resistant Isolates of Enterobacter aerogenes after Biofield Energy Treatment  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 2376-0419 ISBN Medium  
  Area Microbiology Expedition Conference  
  Notes Approved (down) yes  
  Call Number Trivedi Global Inc. @ mahendra @ Serial 42708  
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Author Trivedi, Mahendra Kumar; Branton, Alice; Trivedi, Dahryn; Nayak, Gopal; Mondal, Sambhu Charan; Jana, Snehasis url  doi
openurl 
  Title Evaluation of Antibiogram, Genotype and Phylogenetic Analysis of Biofield Treated Nocardia otitidis Type Journal Article
  Year 2015 Publication Biological Systems: Open Access Abbreviated Journal  
  Volume 4 Issue 2 Pages  
  Keywords Nocardia otitidis; Nocardiosis; Antimicrobial susceptibility; Biofield energy treatment; 16S rDNA sequencing; Random amplified polymorphic DNA  
  Abstract Nocardiosis is a soil-borne aerobic infection caused by Nocardia species commonly affects the respiratory tract. Nocardia otitidis (N. otitidis) is the key organism for non-mycobacterial tuberculosis. The current study was attempted to investigate the effect of Mr. Trivedi’s biofield energy treatment on N. otitidis and analyzed for antimicrobial susceptibility pattern, minimum inhibitory concentration (MIC), DNA polymorphism by Random Amplified Polymorphic DNA (RAPD) and 16S rDNA sequencing. The strain of N. otitidis (ATCC 14630) was divided into two parts, control and treated. Antimicrobial susceptibility was studied using the broth microdilution technique. Overall, the MIC values of 16.67% antimicrobials were changed in the treated group of N. otitidis as compared to the control. Moreover, MIC value of trimethoprim/sulfamethoxazole was reduced by two-fold (0.5/9.5 to 0.25/4.75 μg/mL) in the biofield energy treated sample as compared to the control without alteration in the sensitivity spectrum. The 16S rDNA analysis showed that the treated sample was detected as Enterobacter aerogenes strain NCTC10006T (GenBank Accession No: AJ251468) with 98% identity of gene sequencing data. However, the nearest homolog genus-species was found as Kluyvera cryocrescens (GenBank Accession No: AM184245). Using RAPD biomarkers, the sample showed an average range of 34 to 53% of polymorphism among treated samples as compared to the control. The 16S rDNA sequencing of treated sample was carried out to correlate the phylogenetic relationship of N. otitidis with other bacterial species. These results suggested that Mr. Trivedi’s biofield energy treatment has a significant impact on N. otitidis.  
  Address  
  Corporate Author Thesis  
  Publisher OMICS Publishing Group Place of Publication United States Editor  
  Language English Summary Language English Original Title Evaluation of Antibiogram, Genotype and Phylogenetic Analysis of Biofield Treated Nocardia otitidis  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 2329-6577 ISBN Medium  
  Area Microbiology Expedition Conference  
  Notes Approved (down) yes  
  Call Number Trivedi Global Inc. @ mahendra @ Serial 42710  
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