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Author Gulbahar, O.; Konca Degertekin, C.; Akturk, M.; Yalcin, M.M.; Kalan, I.; Atikeler, G.F.; Altinova, A.E.; Yetkin, I.; Arslan, M.; Toruner, F.
Title A Case With Immunoassay Interferences in the Measurement of Multiple Hormones Type Journal Article
Year 2015 Publication The Journal of Clinical Endocrinology and Metabolism Abbreviated Journal J Clin Endocrinol Metab
Volume 100 Issue 6 Pages (down) 2147-2153
Keywords Adult; Antibodies, Heterophile/*blood; *Artifacts; Cross Reactions; *Diagnostic Techniques, Endocrine/standards; False Positive Reactions; Female; Hormones/*analysis; Humans; Immunoassay/methods; Postpartum Period/blood/immunology
Abstract CONTEXT: Commonly used immunoassays are not free from interference, which can be a confounder in the interpretation of test results. We present a case with extremely high multiple hormone levels due to such interference. CASE DESCRIPTION: A 33-year-old woman with no specific symptoms had markedly elevated TSH with normal free T4 and free T3 levels. Repeated measurements revealed discordantly high TSH, ACTH, FSH, PTH, IGF-1, prolactin, beta-human chorionic gonadotropin, and calcitonin levels without the associated clinical pictures. The measurements were repeated with the same patient sample on four different analytical platforms using chemiluminescence immunoassays/electrochemiluminescence immunoassays, and the results were divergent on each platform. Serial dilutions of serum samples revealed nonlinearity, suggesting assay interference. All hormonal measurements were in the normal range when heterophile antibody blocking tubes were used. The serum of the patient was then subjected to polyethylene glycol precipitation. The post-polyethylene glycol recovery resulted in hormone levels in the normal range. The patient did not receive any medications and has been under follow-up without any signs and symptoms for 24 months. CONCLUSIONS: This report illustrates a rare case of falsely elevated hormone levels due to assay interference caused by heterophile antibodies. We point out the importance of a close collaboration between clinicians and the laboratory to avoid unnecessary clinical investigations as well as inappropriate treatments.
Address Departments of Biochemistry (O.G., G.F.A.) and Endocrinology and Metabolism (C.K.D., M.A., M.M.Y., I.K., A.E.A., I.Y., M.A., F.T.), Gazi University Faculty of Medicine, 06100 Ankara, Turkey
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Language English Summary Language Original Title
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ISSN 0021-972X ISBN Medium
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Notes PMID:25897621 Approved no
Call Number QEHB @ isla.wootton @ Serial 42160
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Author Saito, Y.; Komatsu, K.
Title Functional Role of NBS1 in Radiation Damage Response and Translesion DNA Synthesis Type Journal Article
Year 2015 Publication Abbreviated Journal Biomolecules
Volume 5 Issue 3 Pages (down) 1990--2002
Keywords chemistry,metabolism; Chromatin Assembly and Disassembly,drug effects,radiation effects; DNA,biosynthesis,genetics; DNA Damage; Homologous Recombination,radiation effects; Humans; Nuclear Proteins,metabolism; Radiation; DNA repair; NBS1; chromatin remodeling; homologous recombination; translesion DNA synthesis
Abstract Nijmegen breakage syndrome (NBS) is a recessive genetic disorder characterized by increased sensitivity to ionizing radiation (IR) and a high frequency of malignancies. NBS1, a product of the mutated gene in NBS, contains several protein interaction domains in the N-terminus and C-terminus. The C-terminus of NBS1 is essential for interactions with MRE11, a homologous recombination repair nuclease, and ATM, a key player in signal transduction after the generation of DNA double-strand breaks (DSBs), which is induced by IR. Moreover, NBS1 regulates chromatin remodeling during DSB repair by histone H2B ubiquitination through binding to RNF20 at the C-terminus. Thus, NBS1 is considered as the first protein to be recruited to DSB sites, wherein it acts as a sensor or mediator of DSB damage responses. In addition to DSB response, we showed that NBS1 initiates Polη-dependent translesion DNA synthesis by recruiting RAD18 through its binding at the NBS1 C-terminus after UV exposure, and it also functions after the generation of interstrand crosslink DNA damage. Thus, NBS1 has multifunctional roles in response to DNA damage from a variety of genotoxic agents, including IR.
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ISSN 2218-273x ISBN Medium
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Notes Approved no
Call Number AG @ matthewjvarga @ Serial 47070
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Author Chiolerio, A.; Roppolo, I.; Cauda, V.; Crepaldi, M.; Bocchini, S.; Bejtka, K.; Verna, A.; Pirri, C.F.
Title Ultraviolet mem-sensors: flexible anisotropic composites featuring giant photocurrent enhancement Type Journal Article
Year 2015 Publication Abbreviated Journal Nano Research
Volume 8 Issue 6 Pages (down) 1956-1963
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Notes Cited By :6; Export Date: 2 November 2016 Approved no
Call Number IIT-CSF @ alessandro.chiolerio @ Serial 42778
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Author Andrade-Oliveira, V.; Amano, M.T.; Correa-Costa, M.; Castoldi, A.; Felizardo, R.J.; de Almeida, D.C.; Bassi, E.J.; Moraes-Vieira, P.M.; Hiyane, M.I.; Rodas, A.C.; Peron, J.P.; Aguiar, C.F.; Reis, M.A.; Ribeiro, W.R.; Valduga, C.J.; Curi, R.; Vinolo, M.A.; Ferreira, C.M.; Câmara, N.O.
Title Gut Bacteria Products Prevent AKI Induced by Ischemia-Reperfusion Type Journal Article
Year 2015 Publication Abbreviated Journal J Am Soc Nephrol
Volume 26 Issue 8 Pages (down) 1877-1888
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Abstract Short-chain fatty acids (SCFAs) are fermentation end products produced by the intestinal microbiota and have anti-inflammatory and histone deacetylase-inhibiting properties. Recently, a dual relationship between the intestine and kidneys has been unraveled. Therefore, we evaluated the role of SCFA in an AKI model in which the inflammatory process has a detrimental role. We observed that therapy with the three main SCFAs (acetate, propionate, and butyrate) improved renal dysfunction caused by injury. This protection was associated with low levels of local and systemic inflammation, oxidative cellular stress, cell infiltration/activation, and apoptosis. However, it was also associated with an increase in autophagy. Moreover, SCFAs inhibited histone deacetylase activity and modulated the expression levels of enzymes involved in chromatin modification. In vitro analyses showed that SCFAs modulated the inflammatory process, decreasing the maturation of dendritic cells and inhibiting the capacity of these cells to induce CD4(+) and CD8(+) T cell proliferation. Furthermore, SCFAs ameliorated the effects of hypoxia in kidney epithelial cells by improving mitochondrial biogenesis. Notably, mice treated with acetate-producing bacteria also had better outcomes after AKI. Thus, we demonstrate that SCFAs improve organ function and viability after an injury through modulation of the inflammatory process, most likely via epigenetic modification.
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ISSN 1046-6673 ISBN Medium
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Notes Approved no
Call Number UofT @ mathieu.lemaire @ Serial 45839
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Author Gadagkar, R.
Title How should biologists engage with controversial mathematical theory? Type Journal Article
Year 2015 Publication Abbreviated Journal Current Science
Volume 108 Issue 10 Pages (down) 1869-1873
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Notes Approved no
Call Number CES @ dilipnaidu.gt @ Serial 43034
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Author Borges, R.M.
Title How mutualisms between plants and insects are stabilized Type Journal Article
Year 2015 Publication Abbreviated Journal Current Science
Volume 108 Issue 10 Pages (down) 1862-1868
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Notes Approved no
Call Number CES @ dilipnaidu.gt @ Serial 43028
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Author Karanth, P.K.
Title An island called India: phylogenetic patterns across multiple taxonomic groups reveal endemic radiations Type Journal Article
Year 2015 Publication Abbreviated Journal Current Science
Volume 108 Issue 10 Pages (down) 1847-1851
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Notes Approved no
Call Number CES @ dilipnaidu.gt @ Serial 43063
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Author Sokkar, P.; Boulanger, E.; Thiel, W.; Sanchez-Garcia, E.
Title Hybrid Quantum Mechanics/Molecular Mechanics/Coarse Grained Modeling: A Triple-Resolution Approach for Biomolecular Systems Type Journal Article
Year 2015 Publication Abbreviated Journal J Chem Theory Comput
Volume 11 Issue 4 Pages (down) 1809-1818
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Abstract We present a hybrid quantum mechanics/molecular mechanics/coarse-grained (QM/MM/CG) multiresolution approach for solvated biomolecular systems. The chemically important active-site region is treated at the QM level. The biomolecular environment is described by an atomistic MM force field, and the solvent is modeled with the CG Martini force field using standard or polarizable (pol-CG) water. Interactions within the QM, MM, and CG regions, and between the QM and MM regions, are treated in the usual manner, whereas the CG-MM and CG-QM interactions are evaluated using the virtual sites approach. The accuracy and efficiency of our implementation is tested for two enzymes, chorismate mutase (CM) and p-hydroxybenzoate hydroxylase (PHBH). In CM, the QM/MM/CG potential energy scans along the reaction coordinate yield reaction energies that are too large, both for the standard and polarizable Martini CG water models, which can be attributed to adverse effects of using large CG water beads. The inclusion of an atomistic MM water layer (10 Å for uncharged CG water and 5 Å for polarizable CG water) around the QM region improves the energy profiles compared to the reference QM/MM calculations. In analogous QM/MM/CG calculations on PHBH, the use of the pol-CG description for the outer water does not affect the stabilization of the highly charged FADHOOH-pOHB transition state compared to the fully atomistic QM/MM calculations. Detailed performance analysis in a glycine-water model system indicates that computation times for QM energy and gradient evaluations at the density functional level are typically reduced by 40-70% for QM/MM/CG relative to fully atomistic QM/MM calculations.
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Notes Approved no
Call Number AG @ matthewjvarga @ Serial 46216
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Author Laurenti, M.; Perrone, D.; Verna, A.; Pirri, C.F.; Chiolerio, A.
Title Development of a flexible lead-free piezoelectric transducer for health monitoring in the space environment Type Journal Article
Year 2015 Publication Abbreviated Journal Micromachines
Volume 6 Issue 11 Pages (down) 1729-1744
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Notes Cited By :4; Export Date: 2 November 2016 Approved no
Call Number IIT-CSF @ alessandro.chiolerio @ Serial 42781
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Author Olivera-Couto, A.; Salzman, V.; Mailhos, M.; Digman, M. A.; Gratton, E.; Aguilar, P. S.
Title Eisosomes Are Dynamic Plasma Membrane Domains Showing Pil1-Lsp1 Heteroligomer Binding Equilibrium Type Journal Article
Year 2015 Publication Abbreviated Journal Biophysical Journal
Volume 108 Issue 7 Pages (down) 1633-1644
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Notes Times cited: 14 Approved no
Call Number AG @ matthewjvarga @ Serial 46960
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