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Author Trivedi, Mahendra Kumar; Branton, Alice; Trivedi, Dahryn; Nayak, Gopal; Mondal, Sambhu Charan; Jana, Snehasis url  doi
openurl 
  Title (up) Antibiogram, Biochemical Reactions and Biotyping of Biofield Treated Providencia rettgeri Type Journal Article
  Year 2015 Publication American Journal of Health Research Abbreviated Journal  
  Volume 3 Issue 6 Pages 344-351  
  Keywords Providencia rettgeri; Antimicrobial Sensitivity; Minimum Inhibitory Concentration; Biofield Treatment; Biochemical Reaction; Biotype  
  Abstract Providencia rettgeri (P. rettgeri) is the key organism for gastrointestinal tract infections due to its high virulence properties. The current study was designed to investigate the effect of Mr. Trivedi’s biofield energy treatment on P. rettgeri in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number. The lyophilized strain of P. rettgeri (ATCC 9250) was divided into two parts, Group (Gr.) I: control and Gr. II: treatment. After biofield treatment, Gr. II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzed on day 162 after revival (Study I). The revived sample of Gr. IIB was retreated on day 162 (Study II), and divided into three separate tubes. Tube 1 was analyzed on day 5, likewise, tube 2 and 3 were analyzed on day 10 and 15, respectively after their sub-culturing. All the experimental parameters were studied using automated MicroScan Walk-Away® system. The antimicrobial susceptibility and minimum inhibitory concentration were significantly improved by 71.43%, out of twenty-eight and 56.25%, out of thirty-two, respectively in the treated cells of P. rettgeri as compared to the control. The biochemical reactions also showed the significant (60.61%) alteration in the treated sample with respect to control. The biotype numbers were substantially changed in all the treated groups as compared to the control. Moreover, the organism was changed as Proteus mirabilis in all the treated groups except in Gr. IIA, as compared to the control. These results suggested that biofield treatment has a significant impact on P. rettgeri in lyophilized as well as revived state.  
  Address  
  Corporate Author Thesis  
  Publisher Science Publishing Group Place of Publication United States Editor  
  Language English Summary Language English Original Title Antibiogram, Biochemical Reactions and Biotyping of Biofield Treated Providencia rettgeri  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 2330-8788 (Print) 2330-8796 (Online) ISBN Medium  
  Area Microbiology Expedition Conference  
  Notes Approved yes  
  Call Number Trivedi Global Inc. @ gopal @ Serial 43376  
Permanent link to this record
 

 
Author Trivedi, Mahendra Kumar; Branton, Alice; Trivedi, Dahryn; Nayak, Gopal; Mondal, Sambhu Charan; Jana, Snehasis url  doi
openurl 
  Title (up) Antibiogram, Biochemical Reactions and Genotyping Characterization of Biofield Treated Staphylococcus aureus Type Journal Article
  Year 2015 Publication American Journal of BioScience Abbreviated Journal  
  Volume 3 Issue 6 Pages  
  Keywords Staphylococci; Staphylococcus aureus; Antimicrobial Sensitivity; Biofield Treatment; Biochemical Reaction; Biotype; 16S rDNA; Gram-Positive Bacteria  
  Abstract Staphylococcus aureus (S. aureus) is the key organism for food poisoning due to massive production of heat stable exotoxins. The current study was attempted to investigate the effect of Mr. Trivedi’s biofield treatment on S. aureus. S. aureus (ATCC 25923) was divided into two parts, Group (Gr.) I: control and Gr. II: treatment. After biofield treatment, Gr. II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzed on day 159 after revival (Study I). The revived sample (Gr. IIB) were retreated on day 159 (Study II), and divided into three separate tubes. Tube 1 was analyzed on day 5, likewise, tube 2 and 3 were analyzed on day 10 and 15, respectively. All the experimental parameters were studied using automated MicroScan Walk-Away® system. The 16S rDNA sequencing was carried out in Gr. IIA sample to correlate the phylogenetic relationship of S. aureus with other bacterial species. The antimicrobial susceptibility and minimum inhibitory concentration showed significant alteration i.e. 92.86% and 90.00% respectively in treated cells of S. aureus as compared to control. The biochemical reactions also showed the significant (35.71%) alteration in treated sample with respect to control. The biotype number and microbial species were substantially changed in Gr. IIA (767177; Staphylococcus cohnii subsp. urealyticum) on day 10, while only the biotype numbers were changed in rest of the treated samples as compared to control (307016; S. aureus). The 16S rDNA analysis showed that the identified strain in this experiment was S. aureus (GenBank Accession No.: L37597) after biofield treatment. However, the nearest homolog genus-species was found as Staphylococcus simiae (GenBank Accession No.: DQ127902). These results suggested that biofield treatment has a significant impact on S. aureus in lyophilized as well as revived state.  
  Address  
  Corporate Author Thesis  
  Publisher Science Publishing Group Place of Publication United States Editor  
  Language English Summary Language English Original Title Antibiogram, Biochemical Reactions and Genotyping Characterization of Biofield Treated Staphylococcus aureus  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 2330-0167 ISBN Medium  
  Area Microbiology Expedition Conference  
  Notes Approved yes  
  Call Number Trivedi Global Inc. @ mahendra @ Serial 42711  
Permanent link to this record
 

 
Author Trivedi, Mahendra Kumar; Branton, Alice; Trivedi, Dahryn; Nayak, Gopal; Mondal, Sambhu Charan; Jana, Snehasis url  doi
openurl 
  Title (up) Antibiogram, Biochemical Reactions and Genotyping Characterization of Biofield Treated Staphylococcus aureus Type Journal Article
  Year 2015 Publication American Journal of Bioscience Abbreviated Journal  
  Volume 3 Issue 6 Pages 212-220  
  Keywords Staphylococci; Staphylococcus aureus; Antimicrobial Sensitivity; Biofield Treatment; Biochemical Reaction; Biotype; 16S rDNA; Gram-Positive Bacteria  
  Abstract Staphylococcus aureus (S. aureus) is the key organism for food poisoning due to massive production of heat stable exotoxins. The current study was attempted to investigate the effect of Mr. Trivedi’s biofield treatment on S. aureus. S. aureus (ATCC 25923) was divided into two parts, Group (Gr.) I: control and Gr. II: treatment. After biofield treatment, Gr. II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzed on day 159 after revival (Study I). The revived sample (Gr. IIB) were retreated on day 159 (Study II), and divided into three separate tubes. Tube 1 was analyzed on day 5, likewise, tube 2 and 3 were analyzed on day 10 and 15, respectively. All the experimental parameters were studied using automated MicroScan Walk-Away® system. The 16S rDNA sequencing was carried out in Gr. IIA sample to correlate the phylogenetic relationship of S. aureus with other bacterial species. The antimicrobial susceptibility and minimum inhibitory concentration showed significant alteration i.e. 92.86% and 90.00% respectively in treated cells of S. aureus as compared to control. The biochemical reactions also showed the significant (35.71%) alteration in treated sample with respect to control. The biotype number and microbial species were substantially changed in Gr. IIA (767177; Staphylococcus cohnii subsp. urealyticum) on day 10, while only the biotype numbers were changed in rest of the treated samples as compared to control (307016; S. aureus). The 16S rDNA analysis showed that the identified strain in this experiment was S. aureus (GenBank Accession No.: L37597) after biofield treatment. However, the nearest homolog genus-species was found as Staphylococcus simiae (GenBank Accession No.: DQ127902). These results suggested that biofield treatment has a significant impact on S. aureus in lyophilized as well as revived state.  
  Address  
  Corporate Author Thesis  
  Publisher Science Publishing Group Place of Publication United States Editor  
  Language English Summary Language English Original Title Antibiogram, Biochemical Reactions and Genotyping Characterization of Biofield Treated Staphylococcus aureus  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 2330-0159 (print); 2330-0167 (online) ISBN Medium  
  Area Biotechnology Expedition Conference  
  Notes Approved yes  
  Call Number Trivedi Global Inc. @ dahryn @ Serial 43171  
Permanent link to this record
 

 
Author Trivedi, Mahendra Kumar; Branton, Alice; Trivedi, Dahryn; Nayak, Gopal; Mondal, Sambhu Charan; Jana, Snehasis url  doi
openurl 
  Title (up) Antibiogram, Biochemical Reactions and Genotyping Characterization of Biofield Treated Staphylococcus aureus Type Journal Article
  Year 2015 Publication American Journal of BioScience Abbreviated Journal  
  Volume 3 Issue 6 Pages 212-220  
  Keywords Staphylococci; Staphylococcus aureus; Antimicrobial Sensitivity; Biofield Treatment; Biochemical Reaction; Biotype; 16S rDNA; Gram-Positive Bacteria  
  Abstract Staphylococcus aureus (S. aureus) is the key organism for food poisoning due to massive production of heat stable exotoxins. The current study was attempted to investigate the effect of Mr. Trivedi’s biofield treatment on S. aureus. S. aureus (ATCC 25923) was divided into two parts, Group (Gr.) I: control and Gr. II: treatment. After biofield treatment, Gr. II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzed on day 159 after revival (Study I). The revived sample (Gr. IIB) were retreated on day 159 (Study II), and divided into three separate tubes. Tube 1 was analyzed on day 5, likewise, tube 2 and 3 were analyzed on day 10 and 15, respectively. All the experimental parameters were studied using automated MicroScan Walk-Away® system. The 16S rDNA sequencing was carried out in Gr. IIA sample to correlate the phylogenetic relationship of S. aureus with other bacterial species. The antimicrobial susceptibility and minimum inhibitory concentration showed significant alteration i.e. 92.86% and 90.00% respectively in treated cells of S. aureus as compared to control. The biochemical reactions also showed the significant (35.71%) alteration in treated sample with respect to control. The biotype number and microbial species were substantially changed in Gr. IIA (767177; Staphylococcus cohnii subsp. urealyticum) on day 10, while only the biotype numbers were changed in rest of the treated samples as compared to control (307016; S. aureus). The 16S rDNA analysis showed that the identified strain in this experiment was S. aureus (GenBank Accession No.: L37597) after biofield treatment. However, the nearest homolog genus-species was found as Staphylococcus simiae (GenBank Accession No.: DQ127902). These results suggested that biofield treatment has a significant impact on S. aureus in lyophilized as well as revived state.  
  Address  
  Corporate Author Thesis  
  Publisher Science Publishing Group Place of Publication United States Editor  
  Language English Summary Language English Original Title Antibiogram, Biochemical Reactions and Genotyping Characterization of Biofield Treated Staphylococcus aureus  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 2330-0159 (print) 2330-0167 (online) ISBN Medium  
  Area Biotechnology Expedition Conference  
  Notes Approved yes  
  Call Number Trivedi Global Inc. @ gopal @ Serial 43186  
Permanent link to this record
 

 
Author Trivedi, Mahendra Kumar; Branton, Alice; Trivedi, Dahryn; Nayak, Gopal; Mondal, Sambhu Charan; Jana, Snehasis url  doi
openurl 
  Title (up) Antibiogram, Biochemical Reactions and Genotyping Characterization of Biofield Treated Staphylococcus aureus Type Journal Article
  Year 2015 Publication American Journal of Bioscience Abbreviated Journal  
  Volume 3 Issue 6 Pages 212-220  
  Keywords Staphylococci, Staphylococcus aureus, Antimicrobial Sensitivity, Biofield Treatment, Biochemical Reaction, Biotype, 16S rDNA, Gram-Positive Bacteria  
  Abstract Staphylococcus aureus (S. aureus) is the key organism for food poisoning due to massive production of heat stable exotoxins. The current study was attempted to investigate the effect of Mr. Trivedi’s biofield treatment on S. aureus. S. aureus (ATCC 25923) was divided into two parts, Group (Gr.) I: control and Gr. II: treatment. After biofield treatment, Gr. II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzed on day 159 after revival (Study I). The revived sample (Gr. IIB) were retreated on day 159 (Study II), and divided into three separate tubes. Tube 1 was analyzed on day 5, likewise, tube 2 and 3 were analyzed on day 10 and 15, respectively. All the experimental parameters were studied using automated MicroScan Walk-Away® system. The 16S rDNA sequencing was carried out in Gr. IIA sample to correlate the phylogenetic relationship of S. aureus with other bacterial species. The antimicrobial susceptibility and minimum inhibitory concentration showed significant alteration i.e. 92.86% and 90.00% respectively in treated cells of S. aureus as compared to control. The biochemical reactions also showed the significant (35.71%) alteration in treated sample with respect to control. The biotype number and microbial species were substantially changed in Gr. IIA (767177; Staphylococcus cohnii subsp. urealyticum) on day 10, while only the biotype numbers were changed in rest of the treated samples as compared to control (307016; S. aureus). The 16S rDNA analysis showed that the identified strain in this experiment was S. aureus (GenBank Accession No.: L37597) after biofield treatment. However, the nearest homolog genus-species was found as Staphylococcus simiae (GenBank Accession No.: DQ127902). These results suggested that biofield treatment has a significant impact on S. aureus in lyophilized as well as revived state.  
  Address  
  Corporate Author Thesis  
  Publisher Science Publishing Group Place of Publication United States Editor  
  Language English Summary Language English Original Title Antibiogram, Biochemical Reactions and Genotyping Characterization of Biofield Treated Staphylococcus aureus  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 2330-0159 (print) 2330-0167 (online) ISBN Medium  
  Area Biotechnology Expedition Conference  
  Notes Approved yes  
  Call Number Trivedi Global Inc. @ alice @ Serial 43212  
Permanent link to this record
 

 
Author Trivedi, Mahendra Kumar; Branton, Alice; Trivedi, Dahryn; Nayak, Gopal; Gangwar, Mayank; Jana, Snehasis url  doi
openurl 
  Title (up) Antibiogram, Biochemical Reactions, and Genotypic Pattern of Biofield Treated Pseudomonas aeruginosa Type Journal Article
  Year 2015 Publication Journal of Tropical Diseases Abbreviated Journal  
  Volume 4 Issue 1 Pages  
  Keywords Pseudomonas aeruginosa; Biofield energy treatment; Antibiogram; Polymorphism; RAPD; 16S rDNA analysis  
  Abstract Introduction: Complementary and alternative medicine such as biofield energy therapies are highly popular in biomedical health care. The study evaluates the impact of Mr. Trivedi’s biofield energy treatment on Pseudomonas aeruginosa (P. aeruginosa) to evaluate its phenotypic and genotypic characteristics.

Methods: P. aeruginosa ATCC 10145 (American Type Culture Collection) was procured from Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to biofield treatment and analyzed for antibiogram, biochemical reactions, and biotype number using automated MicroScan Walk-Away® system on day 10. The treated sample was evaluated for DNA polymorphism by Random Amplified Polymorphic DNA (RAPD) and 16S rDNA sequencing to establish the phylogenetic relationship, the epidemiological relatedness and genetic characteristics.

Results: Data showed altered sensitivity pattern in antibiotic cefotaxime from intermediate to decreased β-lactamases activity, with four-fold decreased minimum inhibitory concentration (MIC), i.e. 32 to ≤8 µg/mL as compared to control. Similarly, cefotetan and extended-spectrum-β-lactamases (ESBL-b Scrn) showed decrease in MIC values as compared to the control group. Nitrate reported for negative biochemical reaction i.e. positive (+) to negative (-) after biofield treatment on P. aeruginosa. The biotyping showed a change in biotype number (02063722) as compared to the control (02063726), without altering the microorganism. RAPD analysis showed an average range of 30 to 50% of polymorphism, while 16S rDNA sequencing analyzed treated sample as Pseudomonas aeruginosa (GenBank Accession Number: EU090892) with 99% identity of gene sequencing data.

Conclusion: These results suggest that Mr. Trivedi’s unique biofield energy treatment on P. aeruginosa has an impact to alter the antimicrobial sensitivity pattern and MIC values, thus it can be used as an alternate integrative approach of energy medicine in near future.
 
  Address  
  Corporate Author Thesis  
  Publisher Omics Publishing Group Place of Publication United States Editor  
  Language English Summary Language English Original Title Antibiogram, Biochemical Reactions, and Genotypic Pattern of Biofield Treated Pseudomonas aeruginosa  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 2329-891X ISBN Medium  
  Area Microbiology Expedition Conference  
  Notes Approved yes  
  Call Number Trivedi Global Inc. @ mahendra @ Serial 42918  
Permanent link to this record
 

 
Author Trivedi, Mahendra Kumar; Branton, Alice; Trivedi, Dahryn; Nayak, Gopal; Gangwar, Mayank; Jana, Snehasis url  doi
openurl 
  Title (up) Antibiogram, Biochemical Reactions, and Genotypic Pattern of Biofield Treated Pseudomonas aeruginosa Type Journal Article
  Year 2015 Publication Journal of Tropical Diseases Abbreviated Journal  
  Volume 4 Issue 1 Pages  
  Keywords Pseudomonas aeruginosa; Biofield energy treatment; Antibiogram; Polymorphism; RAPD; 16S rDNA analysis  
  Abstract Introduction: Complementary and alternative medicine such as biofield energy therapies are highly popular in biomedical health care. The study evaluates the impact of Mr. Trivedi’s biofield energy treatment on Pseudomonas aeruginosa (P. aeruginosa) to evaluate its phenotypic and genotypic characteristics.

Methods: P. aeruginosa ATCC 10145 (American Type Culture Collection) was procured from Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to biofield treatment and analyzed for antibiogram, biochemical reactions, and biotype number using automated MicroScan Walk-Away® system on day 10. The treated sample was evaluated for DNA polymorphism by Random Amplified Polymorphic DNA (RAPD) and 16S rDNA sequencing to establish the phylogenetic relationship, the epidemiological relatedness and genetic characteristics.

Results: Data showed altered sensitivity pattern in antibiotic cefotaxime from intermediate to decreased β-lactamases activity, with four-fold decreased minimum inhibitory concentration (MIC), i.e. 32 to ≤8 µg/mL as compared to control. Similarly, cefotetan and extended-spectrum-β-lactamases (ESBL-b Scrn) showed decrease in MIC values as compared to the control group. Nitrate reported for negative biochemical reaction i.e. positive (+) to negative (-) after biofield treatment on P. aeruginosa. The biotyping showed a change in biotype number (02063722) as compared to the control (02063726), without altering the microorganism. RAPD analysis showed an average range of 30 to 50% of polymorphism, while 16S rDNA sequencing analyzed treated sample as Pseudomonas aeruginosa (GenBank Accession Number: EU090892) with 99% identity of gene sequencing data.

Conclusion: These results suggest that Mr. Trivedi’s unique biofield energy treatment on P. aeruginosa has an impact to alter the antimicrobial sensitivity pattern and MIC values, thus it can be used as an alternate integrative approach of energy medicine in near future.
 
  Address  
  Corporate Author Thesis  
  Publisher Omics Publishing Group Place of Publication United States Editor  
  Language English Summary Language English Original Title Antibiogram, Biochemical Reactions, and Genotypic Pattern of Biofield Treated Pseudomonas aeruginosa  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 2329-891X ISBN Medium  
  Area Microbial Genetics Expedition Conference  
  Notes Approved yes  
  Call Number Trivedi Global Inc. @ dahryn @ Serial 43530  
Permanent link to this record
 

 
Author Trivedi, Mahendra Kumar; Branton, Alice; Trivedi, Dahryn; Nayak, Gopal; Gangwar, Mayank; Jana, Snehasis url  doi
openurl 
  Title (up) Antibiogram, Biochemical Reactions, and Genotypic Pattern of Biofield Treated Pseudomonas aeruginosa Type Journal Article
  Year 2015 Publication Journal of Tropical Diseases Abbreviated Journal  
  Volume 4 Issue 1 Pages  
  Keywords Pseudomonas aeruginosa; Biofield energy treatment; Antibiogram; Polymorphism; RAPD; 16S rDNA analysis  
  Abstract Introduction: Complementary and alternative medicine such as biofield energy therapies are highly popular in biomedical health care. The study evaluates the impact of Mr. Trivedi’s biofield energy treatment on Pseudomonas aeruginosa (P. aeruginosa) to evaluate its phenotypic and genotypic characteristics.

Methods: P. aeruginosa ATCC 10145 (American Type Culture Collection) was procured from Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to biofield treatment and analyzed for antibiogram, biochemical reactions, and biotype number using automated MicroScan Walk-Away® system on day 10. The treated sample was evaluated for DNA polymorphism by Random Amplified Polymorphic DNA (RAPD) and 16S rDNA sequencing to establish the phylogenetic relationship, the epidemiological relatedness and genetic characteristics.

Results: Data showed altered sensitivity pattern in antibiotic cefotaxime from intermediate to decreased β-lactamases activity, with four-fold decreased minimum inhibitory concentration (MIC), i.e. 32 to ≤8 µg/mL as compared to control. Similarly, cefotetan and extended-spectrum-β-lactamases (ESBL-b Scrn) showed decrease in MIC values as compared to the control group. Nitrate reported for negative biochemical reaction i.e. positive (+) to negative (-) after biofield treatment on P. aeruginosa. The biotyping showed a change in biotype number (02063722) as compared to the control (02063726), without altering the microorganism. RAPD analysis showed an average range of 30 to 50% of polymorphism, while 16S rDNA sequencing analyzed treated sample as Pseudomonas aeruginosa (GenBank Accession Number: EU090892) with 99% identity of gene sequencing data.

Conclusion: These results suggest that Mr. Trivedi’s unique biofield energy treatment on P. aeruginosa has an impact to alter the antimicrobial sensitivity pattern and MIC values, thus it can be used as an alternate integrative approach of energy medicine in near future.
 
  Address  
  Corporate Author Thesis  
  Publisher Omics Publishing Group Place of Publication United States Editor  
  Language English Summary Language English Original Title Antibiogram, Biochemical Reactions, and Genotypic Pattern of Biofield Treated Pseudomonas aeruginosa  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 2329-891X ISBN Medium  
  Area Microbial Genetics Expedition Conference  
  Notes Approved yes  
  Call Number Trivedi Global Inc. @ alice @ Serial 43579  
Permanent link to this record
 

 
Author Trivedi, Mahendra Kumar; Branton, Alice; Trivedi, Dahryn; Nayak, Gopal; Gangwar, Mayank; Jana, Snehasis url  doi
openurl 
  Title (up) Antibiogram, Biochemical Reactions, and Genotypic Pattern of Biofield Treated Pseudomonas aeruginosa Type Journal Article
  Year 2015 Publication Journal of Tropical Diseases Abbreviated Journal  
  Volume 4 Issue 1 Pages  
  Keywords Pseudomonas aeruginosa; Biofield energy treatment; Antibiogram; Polymorphism; RAPD; 16S rDNA analysis  
  Abstract Introduction: Complementary and alternative medicine such as biofield energy therapies are highly popular in biomedical health care. The study evaluates the impact of Mr. Trivedi’s biofield energy treatment on Pseudomonas aeruginosa (P. aeruginosa) to evaluate its phenotypic and genotypic characteristics.

Methods: P. aeruginosa ATCC 10145 (American Type Culture Collection) was procured from Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to biofield treatment and analyzed for antibiogram, biochemical reactions, and biotype number using automated MicroScan Walk-Away® system on day 10. The treated sample was evaluated for DNA polymorphism by Random Amplified Polymorphic DNA (RAPD) and 16S rDNA sequencing to establish the phylogenetic relationship, the epidemiological relatedness and genetic characteristics.

Results: Data showed altered sensitivity pattern in antibiotic cefotaxime from intermediate to decreased β-lactamases activity, with four-fold decreased minimum inhibitory concentration (MIC), i.e. 32 to ≤8 µg/mL as compared to control. Similarly, cefotetan and extended-spectrum-β-lactamases (ESBL-b Scrn) showed decrease in MIC values as compared to the control group. Nitrate reported for negative biochemical reaction i.e. positive (+) to negative (-) after biofield treatment on P. aeruginosa. The biotyping showed a change in biotype number (02063722) as compared to the control (02063726), without altering the microorganism. RAPD analysis showed an average range of 30 to 50% of polymorphism, while 16S rDNA sequencing analyzed treated sample as Pseudomonas aeruginosa (GenBank Accession Number: EU090892) with 99% identity of gene sequencing data.

Conclusion: These results suggest that Mr. Trivedi’s unique biofield energy treatment on P. aeruginosa has an impact to alter the antimicrobial sensitivity pattern and MIC values, thus it can be used as an alternate integrative approach of energy medicine in near future.
 
  Address  
  Corporate Author Thesis  
  Publisher Omics Publishing Group Place of Publication United States Editor  
  Language English Summary Language English Original Title Antibiogram, Biochemical Reactions, and Genotypic Pattern of Biofield Treated Pseudomonas aeruginosa  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 2329-891X ISBN Medium  
  Area Microbial Genetics Expedition Conference  
  Notes Approved yes  
  Call Number Trivedi Global Inc. @ gopal @ Serial 43590  
Permanent link to this record
 

 
Author Trivedi, Mahendra Kumar; Patil, Shrikant; Shettigar, Harish; Gangwar, Mayank; Jana, Snehasis url  doi
openurl 
  Title (up) Antimicrobial Sensitivity Pattern of Pseudomonas fluorescens after Biofield Treatment Type Journal Article
  Year 2015 Publication Infectious Diseases and Therapy Abbreviated Journal  
  Volume 3 Issue 3 Pages  
  Keywords Pseudomonas fluorescens; Antibiotic susceptibility; Biofield treatment; Biochemical reactions; Biotyping  
  Abstract Global emergence of Pseudomonas fluorescens (P. fluorescens) displays a mechanism of resistance to all existing antimicrobials. Due to its strong ability to acquire resistance, there is a need of some alternative treatment strategy. Objective of this study was to investigate the effect of biofield treatment on antimicrobial sensitivity pattern of P. fluorescens. P. fluorescens cells were procured from MicroBioLogics in sealed packs bearing the American Type Culture Collection (ATCC 49838) number. Two sets of ATCC samples were taken in this experiment and denoted as A and B. ATCC-A sample was revived and divided into two groups (Gr) i.e. Gr.I (control) and Gr.II (revived); likewise, ATCC-B was labeled as Gr.III (lyophilized). Gr.II and III were given biofield treatment and were measured by MicroScan Walk-Away® system before and after treatment. Parameters studied in experiment were antimicrobial sensitivity, minimum inhibitory concentration (MIC), biochemical reactions, and biotype number of both control and treatment groups using MicroScan Walk-Away® system. Experimental results showed antimicrobials such as cefepime, cefotaxime, ceftazidime, ceftriaxone, ciprofloxacin, piperacillin, tetracycline, and tobramycin showed altered sensitivity and MIC values in treated group as compared to control. Biochemical reactions showed positive reaction in malonate, melibiose, nitrate, galactosidase, ornithine, raffinose, sorbitol, sucrose, tobramycin and Voges-Proskauer in Gr.II. Arabinose, colistin, glucose, and rhaminose also showed positive reactions in Gr.II on day 10 while arginine and cetrimide showed negative reaction in Gr.III as compared to control. Biochemical tests results revealed a change in biotype number in Gr.II (34101173, day 5), (77103177, a very rare biotype on day 10) and Gr.III (40000043) as compared to control (02041722). Organism was identified as Enterobacter cloacae (GrII, day 10) and Vibrio fluvialis (Gr.III, day 10) with respect to control. These findings suggest that biofield treatment made significant alteration in sensitivity pattern, MIC values, and biotype number of P. fluorescens.  
  Address  
  Corporate Author Thesis  
  Publisher Omics Publishing Group Place of Publication United States Editor  
  Language English Summary Language English Original Title Antimicrobial Sensitivity Pattern of Pseudomonas fluorescens after Biofield Treatment  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 2332-0877 ISBN Medium  
  Area Microbiology Expedition Conference  
  Notes Approved yes  
  Call Number Trivedi Global Inc. @ mahendra @ Serial 42900  
Permanent link to this record
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