Abstract: The main objective of this study was to evaluate the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health. The test items (viz. vitamin D3 and DMEM), were divided into two parts. One part of each sample received the Consciousness Energy Healing Treatment by Alan Joseph Balmer and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). ALP, collagen, and bone mineralization activities were performed to assess bone health in human bone osteosarcoma cells (MG-63). The test samples (BT) were found as safe in the tested concentrations by MTT assay. ALP was significantly increased by 317.97%, 245.94%, and 247.97% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item, respectively at 10 µg/mL, while increased by 252.87% and 244.05% in the BT-DMEM + BT-Test item at 0.1 and 1 µg/mL, respectively compared to the UT-DMEM + UT-Test item group. Further, the ALP level was significantly elevated by 149.41% and 175.17% in the UT-DMEM + BT-Test item and BT-DMEM + BT-Test item groups, respectively at 100 µg/mL compared to the UT-DMEM + UT-Test item group. Collagen was significantly increased by 607.3%, 346.41%, and 197.61% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 0.1 µg/mL compared to the untreated group. Further, the collagen level was significantly increased by 31.98%, 54.87%, and 67.1% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item and BT-DMEM + BT-Test item, respectively at 100 µg/mL compared to the untreated group. Additionally, the percent of bone mineralization was distinctly increased by 71.41%, 199.92%, and 32.60% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 0.1 µg/mL compared to the untreated group. The percent of bone mineralization was distinctly increased by 28.29% and 25.59% in the UT-DMEM + BT-Test item and BT-DMEM + UT-Test item group at 50 µg/mL, respectively compared to the UT-DMEM + UT-Test item group. Overall, the Biofield Energy Treated vitamin D3 was significantly improved the bone health parameters and it could be a powerful alternative nutraceutical supplement to combat vitamin D3 deficiency and fight against various bone related problems including rickets, osteomalacia, osteoporosis, low bone density, bone, stress management and prevention, autoimmune and inflammatory diseases, and anti-aging by improving overall health.

Abstract: The potential of Consciousness Energy Treated vitamin D3 and DMEM in human bone osteosarcoma cells (MG-63) was investigated. The Test Items (TI), were separated into two parts. One part of each sample was received Biofield Energy Treatment by Joy Angevin Balmer and defined as Biofield Treated (BT) samples, while other parts of each sample were denoted as Untreated Test Items (UT). The cell viability results revealed test samples were found as safe upto 100 µg/mL. ALP was significantly increased by 84.62% in BT-DMEM + BT-TI at 0.1 µg/mL, while increased by 118.24% and 241.62% in UT-DMEM + BT-TI and BT-DMEM + UT-TI, respectively at 1 µg/mL than UT-DMEM + UT-TI. Moreover, ALP was significantly elevated by 43.56% and 192.08% in UT-DMEM + BT-TI and BT-DMEM + UT-TI, respectively at 10 µg/mL than untreated. Collagen was significantly increased by 112.24%, 83.68%, and 142.88% in UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI, respectively at 0.1 µg/mL than untreated. Further, collagen was significantly elevated by 75.39%, 106.15%, and 113.87% in UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI at 1 µg/mL than UT-DMEM + UT-TI. Besides, bone mineralization was remarkably increased by 106.2% and 95.20% at 100 µg/mL in BT-DMEM + UT-TI and BT-DMEM + BT-TI, respectively than untreated. Overall, Biofield Energy Treated vitamin D3 was significantly improved bone health parameters and could be able to fight against various bone-related disorders (osteoporosis, rickets, low bone density, osteogenesis imperfecta), autoimmune and inflammatory diseases, stress, and anti-aging improving overall health.

Abstract: The potential of Consciousness Energy Treated vitamin D3 and DMEM in human bone osteosarcoma cells (MG-63) was investigated. The Test Items (TI), were separated into two parts. One part of each sample was received Biofield Energy Treatment by Joy Angevin Balmer and defined as Biofield Treated (BT) samples, while other parts of each sample were denoted as Untreated Test Items (UT). The cell viability results revealed test samples were found as safe upto 100 µg/mL. ALP was significantly increased by 84.62% in BT-DMEM + BT-TI at 0.1 µg/mL, while increased by 118.24% and 241.62% in UT-DMEM + BT-TI and BT-DMEM + UT-TI, respectively at 1 µg/mL than UT-DMEM + UT-TI. Moreover, ALP was significantly elevated by 43.56% and 192.08% in UT-DMEM + BT-TI and BT-DMEM + UT-TI, respectively at 10 µg/mL than untreated. Collagen was significantly increased by 112.24%, 83.68%, and 142.88% in UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI, respectively at 0.1 µg/mL than untreated. Further, collagen was significantly elevated by 75.39%, 106.15%, and 113.87% in UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI at 1 µg/mL than UT-DMEM + UT-TI. Besides, bone mineralization was remarkably increased by 106.2% and 95.20% at 100 µg/mL in BT-DMEM + UT-TI and BT-DMEM + BT-TI, respectively than untreated. Overall, Biofield Energy Treated vitamin D3 was significantly improved bone health parameters and could be able to fight against various bone-related disorders (osteoporosis, rickets, low bone density, osteogenesis imperfecta), autoimmune and inflammatory diseases, stress, and anti-aging improving overall health.

Abstract: Vitamin D and calcium are one of the important constituents of good bone health. Bone disorders might be to the hormonal factors and lack of proper nutrition that leads to gradual bone loss. The current study aimed to explore the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on various bone health parameters. Vitamin D3 and DMEM medium as test items (TI), were divided into two parts. Each sample received the Consciousness Energy Healing Treatment by Sheila Maureen Benson and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Bone health biomarkers such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization were measured. MTT data showed that cell viability was more than 70% and all the test items were found to be safe at the tested concentrations. ALP level was significantly increased by 135.9% and 134.5% at 1 and 50 µg/mL, respectively in the UT-DMEM+BT-TI, while 84.4%, 93.8%, and 50.0% at 1, 50, and 100 µg/mL, respectively in BT-DMEM+UT-TI group. In addition, BT-DMEM+BT-TI group showed increased ALP by 50.2% and 104.7% at 10 and 50 µg/mL, respectively as compared with the untreated test item and DMEM group. Collagen content was significantly increased by 463.6%, 387.9%, and 239.4% in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI group, respectively at 0.1 µg/mL as compared with the untreated group. Moreover, the percent of bone mineralization was significantly increased by 131.7% and 81.7% at 1 and 10µg/mL, respectively in UT-DMEM+BT-TI group, while 83.1% and 53.6% at 10 and 50µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 152.3% and 108.3% at 50 and 100µg/mL, respectively as compared with the untreated group. Overall, the experimental data suggested that the Biofield Energy Treated vitamin D3 and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. Biofield Energy Treatment might also regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.

Abstract: Vitamin D and calcium are one of the important constituents of good bone health. Bone disorders might be to the hormonal factors and lack of proper nutrition that leads to gradual bone loss. The current study aimed to explore the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on various bone health parameters. Vitamin D3 and DMEM medium as test items (TI), were divided into two parts. Each sample received the Consciousness Energy Healing Treatment by Sheila Maureen Benson and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Bone health biomarkers such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization were measured. MTT data showed that cell viability was more than 70% and all the test items were found to be safe at the tested concentrations. ALP level was significantly increased by 135.9% and 134.5% at 1 and 50 µg/mL, respectively in the UT-DMEM+BT-TI, while 84.4%, 93.8%, and 50.0% at 1, 50, and 100 µg/mL, respectively in BT-DMEM+UT-TI group. In addition, BT-DMEM+BT-TI group showed increased ALP by 50.2% and 104.7% at 10 and 50 µg/mL, respectively as compared with the untreated test item and DMEM group. Collagen content was significantly increased by 463.6%, 387.9%, and 239.4% in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI group, respectively at 0.1 µg/mL as compared with the untreated group. Moreover, the percent of bone mineralization was significantly increased by 131.7% and 81.7% at 1 and 10µg/mL, respectively in UT-DMEM+BT-TI group, while 83.1% and 53.6% at 10 and 50µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 152.3% and 108.3% at 50 and 100µg/mL, respectively as compared with the untreated group. Overall, the experimental data suggested that the Biofield Energy Treated vitamin D3 and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. Biofield Energy Treatment might also regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.